A SARS-CoV-2 Infection Model in Mice Demonstrates Protection by Neutralizing Antibodies.

Cell
Ahmed O HassanMichael S Diamond

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic with millions of human infections. One limitation to the evaluation of potential therapies and vaccines to inhibit SARS-CoV-2 infection and ameliorate disease is the lack of susceptible small animals in large numbers. Commercially available laboratory strains of mice are not readily infected by SARS-CoV-2 because of species-specific differences in their angiotensin-converting enzyme 2 (ACE2) receptors. Here, we transduced replication-defective adenoviruses encoding human ACE2 via intranasal administration into BALB/c mice and established receptor expression in lung tissues. hACE2-transduced mice were productively infected with SARS-CoV-2, and this resulted in high viral titers in the lung, lung pathology, and weight loss. Passive transfer of a neutralizing monoclonal antibody reduced viral burden in the lung and mitigated inflammation and weight loss. The development of an accessible mouse model of SARS-CoV-2 infection and pathogenesis will expedite the testing and deployment of therapeutics and vaccines.

References

Nov 1, 1996·Journal of Virology·N MitterederB C Trapnell
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Nov 23, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Kathleen C F SheehanRobert D Schreiber
Nov 8, 2013·The Journal of General Virology·Christopher M ColemanMatthew B Frieman
Mar 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jincun ZhaoStanley Perlman
Apr 20, 2014·PLoS Pathogens·Amelia K PintoMichael S Diamond
Dec 9, 2014·Cell Host & Microbe·Lulu SunThaddeus S Stappenbeck
Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Davide CortiAntonio Lanzavecchia
Mar 16, 2016·Proceedings of the National Academy of Sciences of the United States of America·Vineet D MenacheryRalph S Baric
Jul 1, 2017·Science Translational Medicine·Timothy P SheahanRalph S Baric
Apr 18, 2019·Immunity·Helen M LazearMichael S Diamond
Jan 25, 2020·The New England Journal of Medicine·Na ZhuUNKNOWN China Novel Coronavirus Investigating and Research Team
Feb 29, 2020·The New England Journal of Medicine·Wei-Jie GuanUNKNOWN China Medical Treatment Expert Group for Covid-19
Mar 14, 2020·Proceedings of the National Academy of Sciences of the United States of America·Shutoku MatsuyamaMakoto Takeda
Apr 7, 2020·Trends in Immunology·Shibo JiangLanying Du
Apr 8, 2020·Cell Host & Microbe·Young-Il KimYoung Ki Choi
Apr 8, 2020·Immunity·Fatima Amanat, Florian Krammer
Apr 11, 2020·American Journal of Clinical Pathology·Lisa M BartonSanjay Mukhopadhyay
Apr 14, 2020·JAMA : the Journal of the American Medical Association·James M SandersJames B Cutrell
Apr 18, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Cynthia MagroJeffrey Laurence
Apr 23, 2020·Cell Host & Microbe·Evangelos J Giamarellos-BourboulisAntonia Koutsoukou
May 6, 2020·Nature Communications·Chunyan WangBerend-Jan Bosch
May 8, 2020·Nature·Linlin BaoChuan Qin
May 31, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jasper Fuk-Woo ChanKwok-Yung Yuen

❮ Previous
Next ❯

Citations

May 22, 2020·European Journal of Immunology·Huibin LvChris K P Mok
Aug 5, 2020·The Journal of Experimental Medicine·Benjamin IsraelowAkiko Iwasaki
Jun 28, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wafaa B AlsoussiAli H Ellebedy
Aug 28, 2020·Open Biology·Vanessa WaznyChristine Cheung
Sep 3, 2020·Journal of Biological Rhythms·Shaon SenguptaGarret A FitzGerald
Jul 30, 2020·The American Journal of Tropical Medicine and Hygiene·Gregory Brett MoreauBarbara J Mann
Apr 18, 2020·The Veterinary Record·Xiangdong Li
Sep 24, 2020·Nature·Florian Krammer
Aug 14, 2020·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Jennifer L WelchJack T Stapleton
Aug 21, 2020·Mucosal Immunology·M D JohansenP M Hansbro
Jul 31, 2020·Nature Reviews. Immunology·Zeyu Chen, E John Wherry
Oct 1, 2020·Scientific Reports·Yuan ZhaoXiaozhong Peng
Aug 21, 2020·Signal Transduction and Targeted Therapy·Shuaiyao LuXiaozhong Peng
Oct 2, 2020·The Journal of Experimental Medicine·Jack Major, Andreas Wack
Sep 6, 2020·Disease Models & Mechanisms·Sarah R LeistDavid R Martinez
Aug 28, 2020·Nature·Kenneth H DinnonRalph S Baric
Sep 24, 2020·Nature·César Muñoz-FontelaDan H Barouch
Oct 22, 2020·Environmental Chemistry Letters·Han DaiEric Lichtfouse
Sep 8, 2020·Clinical Chemistry·Mei San TangChristopher W Farnsworth
Nov 4, 2020·British Journal of Pharmacology·Simon J ClearyClive P Page
Oct 20, 2020·Emerging Microbes & Infections·Raveen RathnasingheMelissa B Uccellini
Dec 1, 2020·Emerging Microbes & Infections·Kyle RosenkeHeinz Feldmann
Dec 2, 2020·Nature Communications·Fatai S OladunniJordi B Torrelles
Dec 2, 2020·Drugs in R&D·Million A TegengeBasil Golding
Jan 4, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Stephany BeyerstedtÉrika Bevilaqua Rangel
Nov 11, 2020·American Journal of Respiratory Cell and Molecular Biology·James A Harker, Cecilia Johansson
Jan 8, 2021·Cell·Wilfredo F Garcia-BeltranAlejandro B Balazs
Jan 14, 2021·Journal of Biomedical Science·Ioannis P TrougakosMeletios A Dimopoulos
Jan 28, 2021·Cell·Xiu NieTiannan Guo
Dec 29, 2020·Frontiers in Veterinary Science·Mohamed A A MahdyZamzam Ewaida

❮ Previous
Next ❯

Datasets Mentioned

BETA
MN908947
MG772933.1
MG772934.1

Methods Mentioned

BETA
transgenic
ELISA
transfection
PCR
Assay

Software Mentioned

GraphPad Prism
GraphPad
FlowJo
MUSCLE
Prism

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.