Jan 6, 2011

A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

Genome Biology
Sheila FisherChad Nusbaum

Abstract

Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.

Mentioned in this Paper

Buffers
Genome-Wide Association Study
Automation, Laboratory
Abnormal Fragmented Structure
Exons
Genome
Enzymes, antithrombotic
Nucleic Acid Hybridization Procedure
Corn of Toe
Tromethamine

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