A Scalable "Junction Substrate" to Engineer Robust DNA Circuits

Journal of the American Chemical Society
Xianbao SunHaojun Liang

Abstract

Versatile building blocks are essential for building complex and scaled-up DNA circuits. In this study, we propose a conceptually new scalable architecture called a "junction substrate" (J-substrate) that is linked by prepurified double-stranded DNA molecules. As a proof-of-concept, this novel type of substrate has been utilized to build multi-input DNA circuits, offering several advantages over the conventional substrate (referred to as a "linear substrate", L-substrate). First, the J-substrate does not require long DNA strands, thus avoiding significant synthetic errors and costs. Second, the traditional PAGE purification method is technically facilitated to obtain high-purity substrates, whereby the initial leakage is effectively eliminated. Third, the asymptotic leakage is eliminated by introducing the "junction". Finally, circuits with the optimized J-substrate architecture exhibit fast kinetics. We believe that the proposed architecture constitutes a sophisticated chassis for constructing complex circuits.

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Citations

Feb 21, 2019·Chemical Communications : Chem Comm·Mengyi XiongWeihong Tan
Sep 11, 2019·Chemical Communications : Chem Comm·Xiang LiHaojun Liang
Jun 4, 2020·Chemical Communications : Chem Comm·Bing WeiHaojun Liang
Oct 15, 2019·The Journal of Physical Chemistry Letters·Shiyan XiaoDavid J Wales
May 16, 2019·ACS Applied Materials & Interfaces·Bing WeiHaojun Liang
Sep 21, 2019·Journal of the American Chemical Society·Ki Tae KimNicolas Winssinger

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