A scalable microfluidic chamber array for sample-loss-free and bubble-proof sample compartmentalization by simple pipetting.

Lab on a Chip
Baobao LinPeng Liu

Abstract

Sample compartmentalization is a pivotal technique in many bioanalytical applications, such as multiplex polymerase chain reaction (PCR) and digital PCR (dPCR). In this study, we successfully developed a novel self-compartmentalization device containing an array of microchambers, each of which is connected to a main microchannel with three capillary burst valves (CBVs) for fluid switching and partitioning. As these CBVs can be automatically opened in a predefined sequence, an incoming solution can be spontaneously directed into the chamber and held in place without further mixing. After that, either air or oil can be loaded into the main channel to isolate each chamber completely. By optimizing the relative burst pressures of the CBVs, a 100% sample utilization rate can be achieved even using a manual pipette and air bubbles in the sample cannot interfere with the loading. In addition, the number of the microchambers in an array can be easily scaled from a few to tens of thousands. To verify the feasibility of this self-compartmentalization method, we successfully conducted mock multiplex loop-mediated isothermal amplifications (LAMP) in an array that contains 144 microchambers, proving that our design method will provide a rob...Continue Reading

References

Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·B Vogelstein, K W Kinzler
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Dec 5, 2006·Journal of Colloid and Interface Science·Hansang ChoTae Song Kim
Mar 29, 2008·Lab on a Chip·Irena Barbulovic-NadAaron R Wheeler
Nov 22, 2008·Lab on a Chip·Ramakrishna SistaVamsee Pamula
Jul 29, 2009·Lab on a Chip·Wenbin DuRustem F Ismagilov
Jun 17, 2010·Analytical Chemistry·Dawn E CohenDaniel T Chiu
Jul 3, 2010·Lab on a Chip·Feng ShenRustem F Ismagilov
Nov 1, 2011·Analytical Chemistry·Benjamin J HindsonBill W Colston
Mar 9, 2012·Lab on a Chip·Alexander GansenDaniel T Chiu
Jul 21, 2012·Lab on a Chip·Yuli WangNancy L Allbritton
Sep 19, 2012·Lab on a Chip·Qiangyuan ZhuYing Mu
Feb 1, 2014·Lab on a Chip·Qiangyuan ZhuYing Mu
Jun 27, 2014·Lab on a Chip·Chaohua ShenWenbin Du
Apr 14, 2017·Analytical Chemistry·Rocio J Jimenez-ValdesJose L Garcia-Cordero
Nov 2, 2017·Lab on a Chip·A J Sposito, D L DeVoe
Nov 15, 2018·Micromachines·Nurhaslina Abd RahmanMarc Madou
Aug 24, 2019·Lab on a Chip·Dmitriy V ZhukovRustem F Ismagilov

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
chip
chips

Software Mentioned

MATLAB

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.