Jan 1, 1982

A schedule to demonstrate radiation-induced sister chromatid exchanges in human lymphocytes

Radiation and Environmental Biophysics
J P Chaudhuri

Abstract

The reciprocal interchange between the chromatids of a chromosome, termed sister chromatid exchange (SCE), is considered to be one of the most sensitive and accurate cytogenetic parameters and respond to toxic chemicals at very low doses. But the response of SCE to ionizing radiation is very poor. Human lymphocytes fail to give SCE response when irradiated at G0. Probably the primary lesions induced at G0 do not remain available long enough to find expression as SCEs. Based on this assumption a schedule was developed using caffeine to demonstrate radiation induced SCEs. Following this schedule a dose-dependent increase in the frequency of radiation induced SCEs has been observed.

Mentioned in this Paper

DRUG Screen Quant Caffeine
Cytogenetics
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Sister Chromatid Exchange
Lymphoid Cells
Dose-Response Relationship, Radiation
Chromatids
Chromosomes
Crossing Over, Genetic
Caffeine Measurement

About this Paper

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