PMID: 9181481Feb 1, 1997Paper

A Schwann cell matrix component of neuromuscular junctions and peripheral nerves

Journal of Neurocytology
S H AstrowC P Ko

Abstract

Molecules localized to the synapse are potential contributors to processes unique to this specialized region, such as synapse formation and maintenance and synaptic transmission. We used an immunohistochemical strategy to uncover such molecules by generating antibodies that selectively stain synaptic regions and then using the antibodies to analyse their antigens. In this study, we utilized a monoclonal antibody, mAb 6D7, to identify and characterize an antigen concentrated at frog neuromuscular junctions and in peripheral nerves. In adult muscle, immunoelectron microscopy indicates that the antigen is located in the extracellular matrix around perisynaptic Schwann cell at the neuromuscular junction and in association with myelinated and nonmyelinated axons in peripheral nerves. The maintenance of the mAb 6D7 epitope is innervation-dependent but is muscle-independent; it disappears from the synaptic region within 2 weeks after denervation, but persists after muscle damage when the nerve is left intact. mAb 6D7 immunolabelling is also detected at the neuromuscular junction in developing tadpoles. Biochemical analyses of nerve extracts indicate that mAb 6D7 recognizes a glycoprotein of 127 kDa with both N- and O-linked carbohydra...Continue Reading

Citations

Sep 10, 2005·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Zhihua FengChien-Ping Ko
Jun 8, 2000·Mechanisms of Development·C Cifuentes-DiazJ P Périn

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