PMID: 7545249Oct 1, 1995Paper

A screen in Escherichia coli for nucleoside analogs that target human immunodeficiency virus (HIV) reverse transcriptase: coexpression of HIV reverse transcriptase and herpes simplex virus thymidine kinase

Journal of Virology
B Kim, L A Loeb

Abstract

Human immunodeficiency virus (HIV) reverse transcriptase substitutes for temperature-sensitive DNA polymerase I (Pol Its) in Escherichia coli, providing a screen for anti-HIV reverse transcriptase nucleoside analogs in bacteria. Since phosphorylation of nucleosides in E. coli is limited to thymidine and its derivatives, we coexpressed herpes simplex virus thymidine kinase, an enzyme that phosphorylates a wide variety of nucleoside analogs, together with HIV reverse transcriptase. Coexpression of herpes simplex virus thymidine kinase and HIV reverse transcriptase rendered Pol Its cells sensitive to dideoxycytidine. Studies with different nucleoside analogs indicate that this bacterial screening system is able to select and identify nucleoside analogs that specifically target HIV reverse transcriptase.

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·V R PrasadS P Goff
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P A FurmanH Mitsuya
Nov 25, 1988·Science·B D PrestonL A Loeb
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·B Kim, L A Loeb
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K M MunirL A Loeb
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J B Sweasy, L A Loeb

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Citations

Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·D V NissleyJ N Strathern
May 20, 2021·International Journal of Antimicrobial Agents·Roberta Maria AntonelloCristina Lagatolla

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