A screening method tuned for mRNA processing factors in human cells by evaluation of the luciferase reporter activity and the subcellular distribution of bulk poly(A)+ RNA

Bioscience, Biotechnology, and Biochemistry
Naoko FujiwaraSeiji Masuda

Abstract

Screening of mRNA export factors in Saccharomyces cerevisiae and Drosophila melanogaster has identified a number of mRNA processing factors involved in multiple mRNA processing steps. However, only limited information is available on human cells. Here we established a screening system searching for mRNA processing factors in human cells by combining the luciferase reporter system and fluorescence in situ hybridization, which evaluates the nuclear/cytoplasmic distribution of bulk poly(A)+ RNA. This system makes it possible to search for the compounds affecting mRNA processing from the various resources.

References

Mar 23, 2002·Cell·George Orphanides, Danny Reinberg
Apr 5, 2002·Nature·Tom Maniatis, Robin Reed
Jul 31, 2003·Molecular Cell·Melissa S Jurica, Melissa J Moore
Nov 25, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Timothy W Nilsen
Dec 4, 2003·Biochimica Et Biophysica Acta·Kiven E Lukong, Stéphane Richard
Feb 11, 2005·Nucleic Acids Research·Suhasini ModemThipparthi R Reddy
Mar 1, 2005·Trends in Genetics : TIG·Miles F Wilkinson
May 20, 2005·Current Opinion in Cell Biology·David L Bentley
Jul 7, 2005·Genes & Development·Seiji MasudaRobin Reed
Jul 20, 2005·Nucleic Acids Research·Wei TanBarbara K Felber
Jun 2, 2006·Molecular and Cellular Biology·Sonia JimenoAndrés Aguilera
Aug 29, 2006·Methods : a Companion to Methods in Enzymology·Papia ChakrabortyBeatriz M A Fontoura
Jan 2, 2007·Experimental Cell Research·Takeyuki SugiuraYuki Nagano
Jun 16, 2007·Biological Chemistry·Saba Valadkhan
Jul 24, 2007·Nature Chemical Biology·Daisuke KaidaMinoru Yoshida
Jul 24, 2007·Nature Chemical Biology·Yoshihiko KotakeYoshiharu Mizui
Sep 6, 2007·Nature Reviews. Molecular Cell Biology·Alwin Köhler, Ed Hurt
Dec 12, 2007·Nature Reviews. Genetics·Suzanne Komili, Pamela A Silver
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patricia ValenciaRobin Reed
Apr 3, 2008·Advances in Experimental Medicine and Biology·Arianne J Matlin, Melissa J Moore
Nov 13, 2008·Structure·Joseph SperlingRuth Sperling
Nov 27, 2008·British Journal of Cancer·R J van AlphenF A L M Eskens
Feb 26, 2009·Cell·Jonathan Houseley, David Tollervey
Jul 21, 2009·Trends in Cell Biology·José Rino, Maria Carmo-Fonseca
Sep 8, 2009·Analytical Biochemistry·Ayan BanerjeeEd Grabczyk
Oct 28, 2009·Genome Biology·Peter J Shepard, Klemens J Hertel

❮ Previous
Next ❯

Citations

Jul 14, 2012·Bioscience, Biotechnology, and Biochemistry·Ken-ichi FujitaSeiji Masuda
Oct 26, 2016·Bioscience, Biotechnology, and Biochemistry·Masashi KurataSeiji Masuda
Mar 9, 2013·The Journal of Clinical Endocrinology and Metabolism·Robert I McLachlan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.