PMID: 8605295Feb 1, 1996Paper

A second and differentially expressed glutamyl-tRNA reductase gene from Arabidopsis thaliana

Plant Molecular Biology
A M KumarD Söll

Abstract

5-Aminolevulinic acid (ALA) is the universal precursor of tetrapyrroles (e.g., chlorophylls and hemes). In the chloroplasts of plants and in several eubacterial species ALA is formed in a two-step process known as the C5 pathway. In the first step, glutamyl-tRNA reductase (GluTR), converts glutamate of glutamyl-tRNA to glutamate 1-semialdehyde (GSA) which is rearranged to ALA by glutamate 1-semialdehyde-2,1-aminomutase (GSA-A) in the second step. Since ALA formation is a limiting step in chlorophyll biosynthesis, GluTR, which is encoded by the HEMA gene in Arabidopsis thaliana plays a vital role in that biosynthesis. Here we report the occurrence of a second functional HEMA gene (HEMA2) in A. thaliana. This gene was isolated by screening a genomic library with a probe from HEMA1. The nucleotide sequence of the cDNA and the corresponding genomic DNA indicates that the Arabidopsis HEMA2 gene contains two short introns (285 bp and 159 bp). The deduced amino acid sequence predicts a HEMA2 protein of 530 amino acids with 79% identity to the HEMA1-encoded GluTR. The 5'-flanking sequence of the HEMA2 gene includes several motifs (e.g., GT-1 boxes, GATA motifs) similar to light-responsive regulatory elements found in light-inducible ge...Continue Reading

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Citations

Apr 20, 2001·The Plant Journal : for Cell and Molecular Biology·A C McCormacM J Terry
Nov 26, 2002·The Plant Journal : for Cell and Molecular Biology·Alex C McCormac, Matthew J Terry
Jan 18, 2007·Annual Review of Plant Biology·Ryouichi Tanaka, Ayumi Tanaka
Mar 11, 2015·Plant Biotechnology Journal·Dilrukshi S K NagahatennaRyan Whitford
Jan 23, 2019·The Plant Journal : for Cell and Molecular Biology·Tiantian ZhiChunmei Ren
Jul 27, 2005·Biochemical Society Transactions·M Moulin, A G Smith
Feb 4, 2012·The Arabidopsis Book·Ryouichi TanakaTatsuru Masuda

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