PMID: 9660241Jul 11, 1998Paper

A second-generation enzyme immunoassay for detection of antibodies to IFN-alpha

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
A V PalleroniJ Raman

Abstract

We have developed a solid-phase enzyme-linked immunoassay (EIA) for detecting antibodies to interferon-alpha2 (IFN-alpha2) in serum or plasma. In this assay, based on the sandwich principle, the capture antigen, IFN-alpha2, is covalently bound to the wells in 96-well plates. This novel procedure offers considerable advantages over the antigen binding by passive adsorption used in most previous EIA. Specific antibodies present in clinical specimens bind to the anchored antigen and are detected by adding peroxidase-labeled IFN-alpha2 and a peroxidase substrate mixture. The resultant color is a function of the concentration of antibody in the sample. The assay has proved to be convenient, precise, and reproducible and can detect as little as 1-5 ng/ml of specific antibody IgG.

References

Jul 1, 1987·Journal of Biological Standardization·U HennesM Rainisio
Nov 1, 1985·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J R QuesadaJ U Gutterman
Dec 31, 1984·Journal of Immunological Methods·W P ProtzmanD K Kelsey

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