A second HD mating type sublocus of Flammulina velutipes is at least di-allelic and active: new primers for identification of HD-a and HD-b subloci

PeerJ
Wei WangBaogui Xie

Abstract

Sexual development in Flammulina velutipes is controlled by two different mating type loci (HD and PR). The HD locus contains homeodomain (Hd) genes on two separate HD subloci: HD-a and HD-b. While the functionality of the HD-b sublocus has been largely confirmed, the status and content of the HD-a sublocus has remained unclear. To examine the function of the HD-a sublocus, genome sequences of a series of F. velutipes strains were analyzed and tested through series of amplification by specific primer sets. Furthermore, activity of di-allelic HD-a locus was confirmed by crossing strains with different combinations of HD-a and HD-b subloci. Sublocus HD-b contained a large variety of fixed Hd1/Hd2 gene pairs, while the HD-a sublocus either contained a conserved Hd2 gene or, a newly discovered Hd1 gene that was also conserved. Identification of whole HD loci, that is, the contents of HD-a and HD-b subloci in a strain, revealed that strains with similar HD-b subloci could still form normal dikaryons if the two genes at the HD-a sublocus differed. At least di-allelic HD-a sublocus, is thus indicated to be actively involved in mating type compatibility. HD-a sublocus is active and di-allelic. Using the new information on the HD subloc...Continue Reading

References

May 24, 1991·Science·A LupasJ Stock
Jun 22, 2001·Trends in Genetics : TIG·A J Brown, L A Casselton
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
May 23, 2007·Nucleic Acids Research·Paul HortonKenta Nakai
Mar 2, 2010·Eukaryotic Cell·Marjatta Raudaskoski, Erika Kothe
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jun 10, 2017·Microbiology Spectrum·Marco A CoelhoTatiana Giraud

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Datasets Mentioned

BETA
KC208594.1
KC208595.2
KU852595.1

Methods Mentioned

BETA
PCR

Software Mentioned

tBLASTn
MUSCLE
MOTIF Search
PSORT
BLASTn
COILS
MEGA
ClustalW
SPAdes3
ZOOMlite

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