A second member of the Nicotiana glauca lipid transfer protein gene family, NgLTP2, encodes a divergent and differentially expressed protein

Functional Plant Biology : FPB
Kimberly D CameronL B Smart

Abstract

Multiple, highly similar members of the lipid transfer protein (LTP) family have been identified in Nicotiana glauca L. Here we describe four new members of the NgLTP gene family and further characterise one member. Three genes were isolated from a guard cell cDNA library and one (NgLTP2) was isolated from a genomic library. These four NgLTPs, as well as one described previously, NgLTP1, share >83% amino acid similarity, but the deduced protein sequence of NgLTP2 lacks the last five residues compared with other LTPs. Since the DNA sequences of the five genes are nearly identical, techniques based on nucleic acid hybridisation or PCR amplification were not sufficient to resolve the expression of the individual genes with confidence. Therefore, we characterised the expression pattern of NgLTP2, the only NgLTP gene that was not found in the guard cell cDNA library, using an NgLTP2 promoter-GUS reporter assay. GUS activity driven by the NgLTP2 promoter was assayed in three species of transgenic plants as an indicator of the endogenous pattern of expression of this gene. GUS was strongly induced upon wounding, whereas NgLTP1 was induced by drought stress. Sequence analysis of the NgLTP2 promoter revealed cis-acting motifs associated...Continue Reading

References

May 1, 1990·Plant Molecular Biology·N HuangR L Rodriguez
Jun 27, 1995·Biochimica Et Biophysica Acta·D P MaJ N Jenkins
Nov 1, 1993·European Journal of Biochemistry·P Coutos-ThevenotJ Guern
Dec 1, 1993·The Plant Journal : for Cell and Molecular Biology·A Molina, F García-Olmedo
Oct 1, 1996·Plant Physiology·S CanevasciniC Kuhlemeier
Jun 1, 1997·The Plant Journal : for Cell and Molecular Biology·P K BuskM Pagès
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Nov 14, 1997·The Plant Journal : for Cell and Molecular Biology·A Molina, F García-Olmedo
Dec 10, 1998·Nucleic Acids Research·K HigoT Korenaga
Feb 19, 1999·BioTechniques·M A Pollock, D G Oppenheimer
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Apr 25, 2000·Plant Science : an International Journal of Experimental Plant Biology·A K KristensenJ D Mikkelsen
Aug 2, 2001·Plant & Cell Physiology·L B SmartA B Bennett
Jun 26, 2002·Plant Molecular Biology·Emmanuel GuiderdoniMichel Delseny

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY621634
AY621632
AY621633
AAG29777
AAF65316
AF228333

Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

BLASTN
MegAlign
PSORT
TBLASTX
DNAstar

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Pharmazeutische Zentralhalle für Deutschland
C PYRIKI
La Presse médicale
Jean-Guillaume LopezLuc de Haro
Chūdoku kenkyū : Chūdoku Kenkyūkai jun kikanshi = The Japanese journal of toxicology
Shinji Funayama
© 2021 Meta ULC. All rights reserved