A second type-I PKS gene cluster isolated from Streptomyces hygroscopicus ATCC 29253, a rapamycin-producing strain

Gene
X RuanL Katz

Abstract

Analysis of a 32.8-kb segment of DNA from the rapamycin (Rp) producer, Streptomyces hygroscopicus ATCC 29253, revealed a new type-I polyketide synthase (PKS) cluster consisting of four open reading frames (ORF 1-4), each encoding a single PKS module. The four ORFs are transcribed in the same direction and are flanked by several smaller ORFs (ORF 5-9), which may be related to the PKS cluster. The first PKS-containing ORF has a ligase domain at the N-terminus of the polypeptide. This domain has 55% aa identity to the CoA ligase domain of the Rp PKS (Schwecke et al., 1995. Proc. Natl. Acad. Sci. 92, 7839-7843) which is also encoded in this strain (Lowden et al., 1996. Angew. Chem. Int. Ed. Engl. 35, 2249-2251). ORF5 (340 aa) and ORF6 (924 aa) were found to be homologous to RapK (41% aa identity) and RapH (35% aa identity), which are hypothesized to be a pteridine-dependent dioxygenase and a regulatory protein, respectively (Molnar et al., 1996. Gene 169, 1-7). In addition, ORF7 (391 aa) was found to have up to 42% aa identity to a number of plant 3-deoxy-D-arabino-heptulosonate-7-phosphate synthases (DAHPS) and 47% aa identity to PhzF, a bacterial DAHPS involved in phenazine antibiotic synthesis. The proximity of the DAHPS-encodin...Continue Reading

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Citations

Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jennifer N AndexerBarrie Wilkinson
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Klaus M. Herrmann, Lisa M. Weaver
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·H IkedaS Omura
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·K A Reynolds
Sep 17, 2013·Journal of Molecular Biology·Puneet JunejaJennifer N Andexer
Nov 5, 1999·Annual Review of Microbiology·R Bentley, J W Bennett
Nov 11, 1999·Medicinal Research Reviews·L Katz, R McDaniel
May 9, 2019·Microorganisms·Chandra RisdianJoachim Wink
Sep 7, 2004·DNA Sequence : the Journal of DNA Sequencing and Mapping·Ross ZirkleIstván Molnár
Apr 16, 2003·Critical Reviews in Biotechnology·Ashish ParadkarDiane Stassi

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