A second Zn(II)(2)Cys(6) transcriptional factor encoded by the YNA2 gene is indispensable for the transcriptional activation of the genes involved in nitrate assimilation in the yeast Hansenula polymorpha

Yeast
Julio AvilaJosé M Siverio

Abstract

Nitrate assimilation genes encoding a nitrate transporter (YNT1), nitrite reductase (YNI1), a Zn(II)(2)Cys(6) transcriptional factor involved in nitrate induction (YNA1) and the nitrate reductase (YNR1) are clustered in the yeast Hansenula polymorpha. A second gene, termed YNA2 (yeast nitrate assimilation), was located seven nucleotides away from the 3' region of YNR1 gene. The cluster is flanked by an ORF encoding a protein with similarity to glutathione-S-transferase on the YNT1 side and an ORF with similarity to Saccharomyces cerevisiae Rad3p on the YNA2 side. The disruption of YNA2 confers the resulting null mutant strain with inability to grow in nitrate. The YNA2 gene encodes a putative protein of 618 residues bearing in the N-terminus the consensus sequence Cys-X(2)-Cys-X(6)-Cys-X(5-16)-Cys-X(2)-Cys-X(6-8)-Cys characteristic of the Zn(II)(2)Cys(6) transcriptional factors. YNA2 is therefore a member of the H. polymorpha nitrate assimilation gene cluster which is transcribed in the opposite direction to the rest of the members. Yna2p shares about 27% similarity with the H. polymorpha Yna1p Zn(II)(2)Cys(6) transcriptional factor involved in nitrate induction. Unlike the wild-type, the yna2::URA3 strain showed no expression ...Continue Reading

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Citations

Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Sarah MacPhersonBernard Turcotte
Feb 14, 2013·Fungal Genetics and Biology : FG & B·Celia RodríguezJosé M Siverio
Oct 11, 2005·FEMS Yeast Research·Beatrice RossiEnrico Berardi
Jul 12, 2002·Yeast
Dec 24, 2013·Eukaryotic Cell·Elisa CabreraJosé M Siverio
May 13, 2014·Applied and Environmental Microbiology·Sam CrauwelsBart Lievens
Aug 7, 2003·The Journal of Biological Chemistry·Adriana Nívea Leao-HelderMarten Veenhuis
Nov 21, 2007·Biochemistry. Biokhimii︠a︡·E V Morozkina, R A Zvyagilskaya
Aug 8, 2002·FEMS Microbiology Reviews·José M Siverio
Sep 6, 2018·Annual Review of Genetics·Hans-Wilhelm NützmannAnne Osbourn
Nov 4, 2020·Yeast·Anjan VenkateshKenneth H Wolfe

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