A secreted luciferase for ex vivo monitoring of in vivo processes.

Nature Methods
Thomas WurdingerBakhos A Tannous

Abstract

Luciferases are widely used to monitor biological processes. Here we describe the naturally secreted Gaussia princeps luciferase (Gluc) as a highly sensitive reporter for quantitative assessment of cells in vivo by measuring its concentration in blood. The Gluc blood assay complements in vivo bioluminescence imaging, which has the ability to localize the signal and provides a multifaceted assessment of cell viability, proliferation and location in experimental disease and therapy models.

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Citations

Apr 6, 2011·Biomedical Microdevices·Andrew W Browne, Chong H Ahn
Mar 13, 2012·Molecular Biotechnology·Salim S El-AmouriDao Pan
Jan 11, 2011·Analytical Chemistry·Johanna M NiersBakhos A Tannous
Dec 14, 2011·Analytical Chemistry·M Sarah S BovenbergBakhos A Tannous
Apr 18, 2012·Analytical Chemistry·M Hannah DegelingBakhos A Tannous
Feb 22, 2013·Analytical Chemistry·M Hannah DegelingBakhos A Tannous
Jul 16, 2009·Analytical Chemistry·Casey A MaguireBakhos A Tannous
Mar 9, 2012·Journal of the American Chemical Society·Johanna M NiersBakhos A Tannous
Jan 28, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hideyuki NakanishiMitsuru Hashida
Mar 5, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bakhos A TannousXandra O Breakefield
Mar 18, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Chunsheng LiuKah-Whye Peng
Feb 24, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Johanna M NiersBakhos A Tannous
Feb 9, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Casey A MaguireJohan Skog
Apr 5, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Danielle Morse, Bakhos A Tannous
Jun 20, 2013·Molecular Therapy. Nucleic Acids·Casey A MaguireBakhos A Tannous
Sep 12, 2013·Nature Communications·Weiqi PanLing Chen
Mar 18, 2010·Nature Methods·Thomas WalterJean-Karim Hériché
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·David P KodackRakesh K Jain
Sep 15, 2010·Assay and Drug Development Technologies·Mieko KatoYoshiro Hanyu
Dec 28, 2010·Assay and Drug Development Technologies·Christian E BadrBakhos A Tannous
May 18, 2013·Assay and Drug Development Technologies·Kerstin HeiseFrank Gaunitz
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Nov 29, 2011·International Journal of Microbiology·Matthias Brock
Apr 8, 2011·Cancer Research·Galina GabrielyAnna M Krichevsky
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Jun 4, 2010·PloS One·Nuria AndreuSiouxsie Wiles
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