PMID: 2119182Jun 1, 1990Paper

A secreted receptor related to M1 protein of Streptococcus pyogenes binds to fibrinogen, IgG, and albumin

Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology
K H Schmidt, T Wadström

Abstract

An extracellular protein (eMP) of Streptococcus pyogenes M type 1 was isolated by affinity chromatography on albumin- and IgG-Sepharose. The protein was found to bind to the human plasma proteins, fibrinogen, IgG, and albumin. Analysis of eMP by Western blotting demonstrated a major band with a molecular weight of 49 kD which was responsible for binding of the three plasma proteins. The purified protein was found to bind selectively to human and primate polyclonal IgG, human and mouse albumin, as well as human fibrinogen which has been the only fibrinogen tested. Serological investigations revealed a close relation of eMP to streptococcal M1 protein. It showed a reaction of identity with cell-extracted M1 protein in immunodiffusion. Moreover, the 49 kD peptide responsible for binding, was recognized with an antiserum directed against the 20 amino acids comprising synthetic peptide (42VAL-61GLU) of the N-terminal part of the M1 protein sequence. The affinity of M protein to plasma proteins other than fibrinogen opens new approaches to its purification by affinity chromatography.

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Citations

May 1, 1995·Medical Microbiology and Immunology·B FleischerK H Schmidt
Jun 1, 1995·Molecular Immunology·M D BoyleA Podbielski
Sep 1, 1995·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·K Sippel, C Lämmler
Oct 1, 1996·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·S I Salasia, C Lämmler
Apr 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·W Tewodros, G Kronvall
Apr 9, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·O KühnemundR Lütticken
Jul 25, 2000·Clinical Microbiology Reviews·M W Cunningham
Oct 18, 2000·Infection and Immunity·M RasmussenL Björck
May 17, 2011·Biochemistry. Biokhimii︠a︡·E V Sidorin, T F Solov'eva
Jun 1, 1994·European Journal of Biochemistry·A Ben NasrU Sjöbring
Oct 25, 1996·The Journal of Biological Chemistry·M de ChâteauL Björck
May 16, 2014·The Journal of Biological Chemistry·Magdalena WisniewskaMats Wikström
Nov 26, 2011·The Journal of Biological Chemistry·Johan MalmströmLars Björck
Aug 1, 1993·FEMS Immunology and Medical Microbiology·K H SchmidtW Köhler
Oct 1, 1993·FEMS Immunology and Medical Microbiology·K H SchmidtW Köhler
Jan 16, 2019·Nature Communications·Simon HauriLars Malmström
May 19, 2000·Methods : a Companion to Methods in Enzymology·U Ringdahl, U Sjöbring
Jul 1, 1995·Clinical and Diagnostic Laboratory Immunology·R Raeder, M D Boyle
Jun 1, 1994·Infection and Immunity·D S Retnoningrum, P P Cleary
May 1, 1992·Infection and Immunity·B FleischerW Köhler

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