A secreted salivary inositol polyphosphate 5-phosphatase from a blood-feeding insect: allosteric activation by soluble phosphoinositides and phosphatidylserine

Biochemistry
J F Andersen, José M C Ribeiro

Abstract

Type II inositol polyphosphate 5-phosphatases (IPPs) act on both soluble inositol phosphate and phosphoinositide substrates. In many cases, these enzymes occur as multidomain proteins in which the IPP domain is linked to lipid-binding or additional catalytic domains. Rhodnius prolixus IPPRp exists as an isolated IPP domain which is secreted into the saliva of this blood-feeding insect. It shows selectivity for soluble and lipid substrates having a 1,4,5-trisphosphate substitution pattern while only poorly hydrolyzing substrates containing a D3 phosphate. With soluble diC8 PI(4,5)P(2) as a substrate, sigmoidal kinetics were observed, suggesting the presence of allosteric activation sites. Surprisingly, IPPRp-mediated hydrolysis of PI(4,5)P(2) and PI(3,4,5)P(3) was also stimulated up to 100-fold by diC8 PI(4)P and diC8 phosphatidylserine (PS). The activation kinetics were again sigmoidal, demonstrating that the allosteric sites recognize nonsubstrate phospholipids. Activation was positively cooperative, and analysis by the Hill equation suggests that at least three to four allosteric sites are present. In a vesicular system, hydrolysis of PI(4,5)P(2) followed a surface dilution kinetic model, and as expected, PS was found to be s...Continue Reading

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Citations

May 6, 2010·Journal of Proteome Research·Ivo M B FrancischettiJosé M C Ribeiro
Nov 28, 2007·BMC Genomics·Rachel BrenchleyLydia Tabernero
Nov 21, 2009·Toxicon : Official Journal of the International Society on Toxinology·John F Andersen
Nov 11, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Hirotomo KatoYoshihisa Hashiguchi
Jan 22, 2008·Insect Biochemistry and Molecular Biology·Teresa C F AssumpçãoJosé M C Ribeiro
Jun 7, 2007·Insect Biochemistry and Molecular Biology·Adriana SantosMarcos Horácio Pereira
Jun 16, 2016·Frontiers in Immunology·Anne GeigerPhilippe Holzmuller
Jan 22, 2015·PloS One·Sean B Johnston, Ronald T Raines
Apr 27, 2017·Journal of Proteomics·María J Hernández-VargasGerardo Corzo
Feb 3, 2020·Trends in Parasitology·Paula B SantiagoJaime M Santana
Jun 9, 2020·Acta Tropica·Daiki MizushimaHirotomo Kato

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