A selective and robust UPLC-MS/MS method for the simultaneous quantitative determination of anlotinib, ceritinib and ibrutinib in rat plasma and its application to a pharmacokinetic study

The Analyst
Ping DuLihong Liu

Abstract

A selective and robust UPLC-MS/MS method has been firstly developed for simultaneous determination of three anti-tumor tyrosine kinase inhibitors (anlotinib, ANL; ceritinib, CER; ibrutinib, IBR) in rat plasma using cost-effective protein precipitation extraction. LC separation was achieved on Waters XBrige C18 column (50 mm × 2.1 mm, 3.5 μm) under gradient conditions in a run time of 5 min. ESI+ was involved through mass spectrometry. Multiple reaction monitoring transitions were at m/z 408.2 → 339.2 for ANL, 558.2 → 433.2 for CER, 441.0 → 138.0 for IBR, 285.0 → 193.1 for diazepam (internal standard), respectively. The optimized method was validated based on US FDA guideline, EMEA guideline as well as Pharmacopoeia of the People's Republic of China. The assay was linear in the range of 0.1-20 ng mL-1 for ANL, 2-1000 ng mL-1 for CER, 1-500 ng mL-1 for IBR. Intra- and inter-day accuracy and precision for all analytes were ≦13.84% and ≦12.56%, respectively. ANL, CER and IBR were sufficiently stable under most investigated conditions. The optimized method was successfully applied for a pharmacokinetic study after single oral gavage administration of mixture (ANL, CER and IBR) at dose of 6 mg kg-1, 25 mg kg-1 and 10 mg kg-1.

References

Mar 8, 2008·Current Opinion in Genetics & Development·Jeffrey A Engelman, Jeffrey Settleman
Oct 30, 2010·Nature Reviews. Drug Discovery·Shivanni KummarJames H Doroshow
Dec 28, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Henning BlumeChristoph Siethoff
Feb 25, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Stéphane BouchetMathieu Molimard
Feb 15, 2012·Cancer Treatment Reviews·Alain Ravaud, Marine Gross-Goupil
Jun 15, 2014·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Nicolas WidmerAlain Astier
Jul 27, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ping DuYuankai Shi
Jan 17, 2015·Journal of Pharmaceutical and Biomedical Analysis·Sridhar VeeraraghavanManivannan Rangasamy
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Aug 2, 2017·ESMO Open·Alexia VogtAurelius Omlin
May 21, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·J J M RoodR W Sparidans
Jun 27, 2018·Drugs·Yahiya Y Syed
Jul 22, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·D BeauvaisC Danel
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

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Citations

Feb 18, 2020·Rapid Communications in Mass Spectrometry : RCM·Mengjie Dai, Guangming Huang
Dec 15, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Irene A RetmanaRolf W Sparidans
Aug 7, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Chunzhan WangHaitao Huang

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