A selective differential medium for Enterobacter sakazakii, a preliminary study

International Journal of Food Microbiology
Carol IversenS J Forsythe

Abstract

Enterobacter sakazakii can cause fatal invasive infection of neonates associated with the presence of this organism in powdered infant milk formula. A new chromogenic medium (Druggan-Forsythe-Iversen agar, DFI) is described for the selective detection of this emergent pathogen. The medium is based on the alpha-glucosidase reaction which is detected using 5-bromo-4-chloro-3-indolyl-alpha,D-glucopyranoside (XalphaGlc). Ent. sakazakii hydrolyses this substrate to an indigo pigment, producing blue-green colonies on this medium. DFI was compared with the current method of detection on violet red bile glucose agar (VRBGA) followed by pigment production on tryptone soy agar (TSA) after 48-72 h at 25 degrees C and subsequent biochemical profile determination using Biomerieux API20E. Ninety-five clinical and food strains of Ent. sakazakii were detected on the DFI chromogenic medium 2 days sooner than the alternative method. The characteristics of 148 strains representing 17 genera of non-Ent. sakazakii Enterobacteriaceae were compared using the two methods. Only 16/18 Escherichia vulneris strains, 2/3 strains of Pantoea spp. and 1/8 Citrobacter koseri strains gave false positive results on DFI agar. Eight alpha-glucosidase positive stra...Continue Reading

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Citations

May 10, 2008·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·M Friedemann
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Dec 5, 2009·Foodborne Pathogens and Disease·Brendan HealySéamus Fanning
Aug 3, 2006·Maternal & Child Nutrition·Stephen J Forsythe
Oct 31, 2006·Applied and Environmental Microbiology·Carol Iversen, Stephen J Forsythe
Mar 4, 2008·Applied and Environmental Microbiology·Carol IversenRoger Stephan
Sep 8, 2006·Applied and Environmental Microbiology·N R MullaneS Fanning
Sep 21, 2007·Journal of Clinical Microbiology·Carol IversenHan Joosten
Nov 6, 2004·Journal of Clinical Microbiology·Carol IversenStephen Forsythe
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Nov 21, 2012·PloS One·Susan JosephStephen J Forsythe
Jul 17, 2014·PloS One·Jinghua CuiZhigang Cui
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Nov 10, 2009·International Journal of Food Microbiology·Patrick Druggan, Carol Iversen
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Sep 5, 2009·International Journal of Food Microbiology·J ChapS J Forsythe
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