A selective gut bacterial bile salt hydrolase alters host metabolism

ELife
Lina YaoA Sloan Devlin

Abstract

The human gut microbiota impacts host metabolism and has been implicated in the pathophysiology of obesity and metabolic syndromes. However, defining the roles of specific microbial activities and metabolites on host phenotypes has proven challenging due to the complexity of the microbiome-host ecosystem. Here, we identify strains from the abundant gut bacterial phylum Bacteroidetes that display selective bile salt hydrolase (BSH) activity. Using isogenic strains of wild-type and BSH-deleted Bacteroides thetaiotaomicron, we selectively modulated the levels of the bile acid tauro-β-muricholic acid in monocolonized gnotobiotic mice. B. thetaiotaomicron BSH mutant-colonized mice displayed altered metabolism, including reduced weight gain and respiratory exchange ratios, as well as transcriptional changes in metabolic, circadian rhythm, and immune pathways in the gut and liver. Our results demonstrate that metabolites generated by a single microbial gene and enzymatic activity can profoundly alter host metabolism and gene expression at local and organism-level scales.

References

Nov 8, 1976·Biochimica Et Biophysica Acta·E J Stellwag, P B Hylemon
Nov 1, 1991·The American Journal of Clinical Nutrition·T TodescoD J Jenkins
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·G BertilssonA Berkenstam
Dec 14, 1999·Journal of Applied Microbiology· De Boever P, W Verstraete
Jun 9, 2000·Annual Review of Neuroscience·D P King, J S Takahashi
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·J L StaudingerS A Kliewer
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 23, 2002·Science·Makoto MakishimaDavid J Mangelsdorf
Nov 8, 2002·The Journal of Biological Chemistry·Gilles LambertChristopher J Sinal
Jan 25, 2003·Annual Review of Biochemistry·David W Russell
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Sep 4, 2004·Applied and Environmental Microbiology·Geun-Bae KimByong H Lee
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fredrik BäckhedJeffrey I Gordon
Feb 22, 2005·Biochemical and Biophysical Research Communications·Susumu KatsumaGozoh Tsujimoto
May 19, 2005·The Journal of Biological Chemistry·Daniel Duran-SandovalBart Staels
Jul 19, 2005·FEBS Letters·Bertrand CariouBart Staels
Nov 22, 2005·Journal of Lipid Research·Jason M RidlonPhillip B Hylemon
Feb 1, 2006·The Journal of Biological Chemistry·Bertrand CariouBart Staels
Mar 7, 2006·Applied and Environmental Microbiology·Máire BegleyCormac G M Gahan
Jul 1, 1995·Applied and Environmental Microbiology·J GrillJ Ballongue
Mar 25, 2006·The Journal of Clinical Investigation·Ke MaDavid D Moore
Dec 22, 2006·Nature·Ruth E LeyJeffrey I Gordon
Dec 22, 2006·Nature·Peter J TurnbaughJeffrey I Gordon
Jan 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Fredrik BäckhedJeffrey I Gordon
Apr 7, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·James P HamiltonAlan F Hofmann
Feb 2, 2008·Nature Reviews. Drug Discovery·Wei JiaJeremy K Nicholson
Mar 26, 2008·Journal of Lipid Research·Erica J ReschlyMatthew D Krasowski
Sep 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Brian V JonesJulian R Marchesi
Feb 11, 2009·The Journal of Biological Chemistry·Theo H van DijkFolkert Kuipers
Jun 6, 2009·Journal of Lipid Research·Paul A DawsonAnuradha Rao
Feb 6, 2010·Genome Biology·Matthew D YoungAlicia Oshlack

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Citations

Jan 17, 2019·The Journal of Biological Chemistry·Irina AlimovXinming Cai
Oct 17, 2019·Nature Reviews. Endocrinology·Tiara R Ahmad, Rebecca A Haeusler
Jan 28, 2020·Microbial Biotechnology·Hui XuLu Cai
May 29, 2020·Pharmacotherapy·Stephanie A FlowersJames C Lee
Jun 7, 2020·Applied and Environmental Microbiology·Jason M Ridlon
Apr 25, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Preeti PathakJ Mark Brown
Apr 25, 2019·Annual Review of Nutrition·John Y L Chiang, Jessica M Ferrell
Jul 26, 2019·Mediators of Inflammation·Caihua WangYuezhong Ren
Apr 2, 2020·Nature Biotechnology·Mao TaketaniChristopher A Voigt
Apr 25, 2020·International Journal of Molecular Sciences·Miranda GreenSatya Prakash
Aug 5, 2020·Nature Chemical Biology·Snehal N ChaudhariA Sloan Devlin
Sep 10, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yunxia Yang, Jianfa Zhang
Jan 13, 2021·Cell Host & Microbe·Snehal N ChaudhariA Sloan Devlin
Jun 16, 2019·EBioMedicine·Hélène C DescampsChristoph A Thaiss
Jan 1, 2021·Journal of the Endocrine Society·Maryan G Rizk, Varykina G Thackray
Jan 26, 2021·Data in Brief·Aleksei A KorzhenkovStepan V Toshchakov
Mar 5, 2020·Cell Metabolism·Emily M TeichmanJohn F Cryan
Jun 17, 2020·Cell Host & Microbe·Douglas J KennyRamnik J Xavier
Mar 9, 2021·Frontiers in Immunology·Haiyan ZhouFeng Liu
Aug 16, 2019·Progress in Biophysics and Molecular Biology·William B MillerFrantišek Baluška
Mar 18, 2021·Microbial Biotechnology·Saeid KhakisahnehDe-Hua Wang
May 18, 2019·The American Journal of Pathology·Joseph M Pickard, Gabriel Núñez
Apr 4, 2021·Microorganisms·Jack W DalyCormac G M Gahan
Apr 16, 2021·Bio-protocol·Snehal N Chaudhari, A Sloan Devlin
May 1, 2021·International Journal of Molecular Sciences·Baolei JiaChe Ok Jeon
Feb 19, 2021·Circulation Research·Huanan ShiDavid J Durgan
Jun 3, 2021·International Journal of Molecular Sciences·Marina Romaní-PérezYolanda Sanz
Jun 3, 2021·Microorganisms·Mélanie BourginMoez Rhimi

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Datasets Mentioned

BETA
GSE112571

Methods Mentioned

BETA
RNA-Seq
blood draw
PCR
ELISA

Software Mentioned

MultiQC
PhyML
CalR
PhyloPhlAn
FastQC
STAR aligner
iTOL
BLASTP
R
goseq

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