A selective medium for Actinobacillus actinomycetemcomitans and the incidence of the organism in juvenile periodontitis

Journal of Periodontology
R L Mandell, S S Socransky

Abstract

A selective medium, malachite green bacitracin agar, was developed for the isolation of Actinobacillus actinomycetemcomitans from subgingival plaque of periodontally diseased patients. The medium consisted of Trypticase soy agar 40 gm/liter, bacitracin 128 micrograms/ml, malachite green 8 micrograms/ml and 5% defibrinated sheep blood. The medium, when incubated in an atmosphere of air plus 10% CO2 for 5 days, permitted greater than 80% recovery of pure cultures of A. actinomycetemcomitans when compared with a nonselective medium. The most frequent contaminant in plaque samples from different clinical conditions was Haemophilus aphrophilus. Decomposition of H2O2 was useful in differentiating these two species. Clinical studies employing the malachite green bacitracin medium revealed a significant association between the presence of the organism, A. actinomycetemcomitans and juvenile periodontitis.

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Citations

May 23, 2009·Clinical Oral Investigations·Isabelle SliepenWim Teughels
Feb 1, 1990·Oral Microbiology and Immunology·K W FriskenJ M Palmer
Apr 1, 1990·Oral Microbiology and Immunology·J M DiRienzoJ Slots
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Mar 1, 1992·Journal of Clinical Periodontology·M D'AngeloF Sammartano
Apr 1, 1997·Journal of Clinical Periodontology·T Larsen, N E Fiehn
May 16, 1998·Journal of Clinical Periodontology·M F TimmermanU Van der Velden
Oct 5, 2001·Clinical Microbiology Reviews·W J Loesche, N S Grossman
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Nov 1, 1996·Annals of Periodontology·J J Zambon
Jun 23, 2000·Annals of Periodontology·M S Tonetti, A Mombelli
Dec 1, 1983·Journal of Periodontology·A C EisenmannJ Slots
Aug 1, 1985·Journal of Periodontology·K S Kornman, P B Robertson
Dec 1, 1988·Journal of Periodontology·J S MinutelloJ Carter
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Dec 10, 1999·Journal of Periodontology·L SbordoneV J Iacono
Jan 28, 2003·Journal of Periodontology·Marc PlombasGilbert C Faure

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