A Selective Medium for Isolation and Detection of Candida auris, an Emerging Pathogen.

Journal of Clinical Microbiology
Sourav DasAnup Ghosh

Abstract

Identification of Candida auris is challenging and requires molecular or protein profiling-based approaches, availability of which is limited in many routine diagnostic laboratories, necessitating the development of a cost-effective, rapid, and reliable method of identification. The objective of this study was to develop a selective medium for C. auris identification. Eighteen C. auris and 30 non-C. auris yeasts were used for the standardization of the selective medium. Sodium chloride (10% to 13% concentration) and ferrous sulfate (8 mM to 15 mM) were added to yeast extract-peptone-dextrose (YPD) agar in various combinations followed by incubation at 37°C, 40°C, or 42°C for 2 to 3 days. For validation, 579 yeast isolates and 40 signal-positive Bactec blood culture (BC) broths were used. YPD agar comprising 12.5% NaCl and 9 mM ferrous sulfate incubated at 42°C for 48 h, named Selective Auris Medium (SAM), allowed selective growth of C. auris A total of 95% (127/133) of C. auris isolates tested grew on the standardized media within 48 h, and the remaining 6 isolates grew after 72 h, whereas the growth of 446 non-C. auris yeast isolates was completely inhibited. The specificity and sensitivity of the test medium were both 100% af...Continue Reading

References

Sep 6, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A ChakrabartiK L N Rao
Feb 7, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A K GhoshA Chakrabarti
Nov 25, 2016·Journal of Clinical Microbiology·Masako MizusawaSean X Zhang
Dec 19, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shawn R LockhartAnastasia P Litvintseva
Jan 19, 2017·Emerging Infectious Diseases·Leighann SherryRiina Rautemaa-Richardson
Mar 24, 2017·The Journal of Antimicrobial Chemotherapy·Shivaprakash M RudramurthyAnup Ghosh
Sep 25, 2017·The Journal of Hospital Infection·M BiswalA Chakrabarti
Nov 17, 2017·Clinical Microbiology Reviews·Anna Jeffery-SmithColin S Brown
Nov 24, 2017·Journal of Clinical Microbiology·Emily S Spivak, Kimberly E Hanson
Mar 6, 2018·Clinical Microbiology Newsletter·Shawn R LockhartRory M Welsh
Jul 4, 2018·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Amanda J DuranteDavid B Banach
Jul 18, 2018·Emerging Infectious Diseases·Shiva Pekard-AmenitschWalter Buzina
Jul 22, 2018·Journal de mycologie médicale·A L BidaudE Dannaoui
Dec 27, 2018·Mycoses·Mahdi AbastabarHamid Badali
Feb 19, 2019·Mycoses·Shabir A Lone, Aijaz Ahmad
May 3, 2019·Critical Care : the Official Journal of the Critical Care Forum·Andrea CortegianiDavid Eyre
May 28, 2019·Mycoses·Rachna SinghShivaprakash M Rudramurthy
Jul 17, 2019·Emerging Infectious Diseases·Nancy A ChowJacques F Meis

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