A selective, nontoxic, OFF-ON fluorescent molecular sensor based on 8-hydroxyquinoline for probing Cd(2+) in living cells

Chemistry : a European Journal
Marta MameliNelsi Zaccheroni

Abstract

In spite of the fact that cadmium(II) has been recognized as a highly toxic element and that excessive exposure to this metal ion has been reported to have many adverse effects on human health, very few selective and specific fluorescent probes are available for imaging Cd(2+) in living cells. Herein, we report the spectroscopic and photochemical characterization of 5-(5-chloro-8-hydroxyquinolinylmethyl)-2,8-dithia-5-aza-2,6-pyridinophane (L) as a fluorescent sensor for the selective imaging of Cd(2+) in living cells. In particular, the response of L to Cd(2+) was first assessed in aqueous solutions, sodium dodecyl sulfate micelles, and liposomes, and subsequently in living cells by fluorescence microscopy techniques. Cytofluorimetric analyses of leukemic HL-60 cells loaded with L also allowed evaluation of the toxicity of the probe and the selective analysis of its intracellular fluorescence in the presence of Cd(2+). Furthermore, the 1:1 complex species [Cd(L)H(2)O](2+) responsible for the OFF-ON chelation enhancement of fluorescence (CHEF) effect on L was structurally characterized; time-dependent DFT calculations allowed the prediction of theoretical excitations, which were comparable with the experimental ones.

References

Aug 1, 1965·Journal of Molecular Biology·A D BanghamJ C Watkins
Mar 18, 2000·Journal of Computer-aided Molecular Design·G Schaftenaar, J H Noordik
Feb 8, 2002·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·D Beyersmann, H Haase
Feb 26, 2004·Journal of the American Chemical Society·Xiangfeng GuoLihua Jia
Aug 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Liuchun YangChristoph J Fahrni
Aug 4, 2005·Journal of the American Chemical Society·Hirokazu KomatsuKoji Suzuki
Jan 5, 2006·Journal of the American Chemical Society·Li ZengChristopher J Chang
Jan 5, 2006·Journal of the American Chemical Society·Giovanna FarruggiaFederica I Wolf
Jun 14, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Marco MontaltiUmberto Tonellato
Jul 20, 2006·Journal of the American Chemical Society·Qiwen HeChristopher J Chang
Oct 26, 2006·Journal of the American Chemical Society·Sung-Kyun KoInjae Shin
Dec 19, 2006·Toxicology Letters·Anna Nordberg, Christina Rudén
Feb 8, 2007·Journal of the American Chemical Society·Xiaojun PengTao Xu
Mar 17, 2007·Journal of Toxicology and Environmental Health. Part a·K G CoonseS J Heggland
Apr 13, 2007·Journal of Chemical Information and Modeling·Karen L SchuchardtTheresa L Windus
Dec 25, 2007·Acta Crystallographica. Section A, Foundations of Crystallography·George M Sheldrick
Jan 8, 2008·Journal of the American Chemical Society·Gregory M CockrellRobert D Hancock
Apr 23, 2008·Chemical Reviews·Emily L QueChristopher J Chang
Jul 26, 2008·Chemical Reviews·Elizabeth M Nolan, Stephen J Lippard
Sep 3, 2008·Journal of the American Chemical Society·Masayasu TakiYukio Yamamoto
Nov 8, 2008·Accounts of Chemical Research·Elizabeth M Nolan, Stephen J Lippard
Nov 14, 2008·Journal of the American Chemical Society·Tanyu ChengLiping Duan
Feb 5, 2009·Journal of the American Chemical Society·Hyo Sung JungJong Seung Kim

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Citations

Mar 13, 2012·Photochemistry and Photobiology·Hannah W MbatiaShawn C Burdette
Nov 3, 2015·Chemical & Pharmaceutical Bulletin·Zhi-Gang SunHai-Liang Zhu
Apr 24, 2012·Biochimica Et Biophysica Acta·Kevin M DeanAmy E Palmer
Aug 30, 2016·Inorganic Chemistry·Sanghamitra SinhaPradyut Ghosh
Jan 31, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Yuji MikataSatoshi Iwatsuki
Aug 27, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·M Carla AragoniStefano Puccioni
Sep 17, 2013·Chemistry : a European Journal·Valentina OliveriJohn Spencer
Apr 25, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Samir MaityAjay Misra
Dec 21, 2011·Chemical Society Reviews·Ha Na KimJuyoung Yoon
Jun 26, 2020·Chembiochem : a European Journal of Chemical Biology·Ivana DrienovskáGerard Roelfes
Jul 30, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Surajit MondalSwapan Dey
Oct 12, 2011·Chemical Communications : Chem Comm·Jan L VinkenborgMaarten Merkx
Apr 14, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Yiming LiQingxiang Guo
Oct 4, 2012·Organic & Biomolecular Chemistry·Alexander CiupaLorenzo Caggiano
May 17, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Li-Kun ZhangLong-Ji Shi
Feb 6, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Chanda KumariSwapan Dey
May 4, 2013·Chemistry, an Asian Journal·Hui He, Dennis K P Ng
Nov 21, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Wei WangXiaoyi Xu
Nov 6, 2020·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]· RohiniVijay Luxami
Dec 3, 2014·Inorganic Chemistry·Xiu-Juan JiangThomas C W Mak
Mar 5, 2014·Chemical Reviews·Kyle P CarterAmy E Palmer
Sep 13, 2011·The Journal of Physical Chemistry. B·Amit K GhoshPrasun Bandyopadhyay
Sep 13, 2011·Inorganic Chemistry·Qiang ZhaoXian-He Bu

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