A Selective Regional Response of Cultured Astrocytes to Methamphetamine(a)

Annals of the New York Academy of Sciences
A StadlinY K Szeto

Abstract

Methamphetamine (METH) has long-lasting neurotoxic effects on the dopamine and forebrain serotonin systems. It was reported that METH would induce the release of glutamate within the striatum and that it also caused astrogliosis. The mechanisms of this release and subsequent neurotoxicity are not well defined. The aim of this study was to examine the response of cultured astrocytes after METH-induced injury. Astrocytes were cultured from neonatal C57B1/6 mice brains. Cells were obtained from the mesencephalon, striatum and cortex in order to examine any regional differences. Cells were treated with 4 mM METH for 4, 8, 12, 24 and 48 hr. Lactate dehydrogenase (LDH) levels were used as a measure of cell viability. At various time points, Western blot analyses were performed to study the change in GFAP and vimentin (markers for astrogliosis) levels. Change in glutamine synthase (GS), the enzyme that catalyzes the synthesis of glutamine from glutamate and ammonia in astrocytes, was also examined. The results showed that METH caused marked astrogliosis in striatal and mesencephalic astrocytes. Cells were transformed from protoplasmic (inactive) to fibrous (reactive) form after 48 hr treatment. There were also large amounts of vacuole...Continue Reading

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Citations

Mar 8, 2018·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Tara L WhiteSinda Fekir
Jan 31, 2017·Forensic Sciences Research·Zhiyong ZhangLi Quan
Jun 21, 2017·The Journal of Pharmacology and Experimental Therapeutics·Anna Moszczynska, Sean Patrick Callan
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Jun 30, 2019·Scientific Reports·Ramesh B BadisaCarl B Goodman

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