PMID: 8446579Mar 1, 1993Paper

A selective transcriptional induction system for mammalian cells based on Gal4-estrogen receptor fusion proteins

Proceedings of the National Academy of Sciences of the United States of America
S BraselmannM Busslinger

Abstract

Most mammalian cells neither express any Gal4-like activity nor endogenous estrogen receptor, thus rendering estrogen an inert signal for them. For these two reasons we have developed a selective induction system based on the estrogen-regulable transcription factor Gal-ER. Gal-ER consists of the DNA-binding domain of the yeast Gal4 protein fused to the hormone-binding domain of the human estrogen receptor and hence should exclusively regulate a transfected gene under the control of a Gal4-responsive promoter in mammalian cells. Two major improvements of this induction system were made. First, a synthetic Gal4-responsive promoter was constructed which consisted of four Gal4-binding sites, an inverted CCAAT element, a TATA box, and the adenovirus major late initiation region. This promoter showed extremely low basal activity in the absence and high inducibility in the presence of ligand-activated Gal-ER. Second, the transcription factor Gal-ER was rendered more potent and less susceptible to cell type-specific variation by fusing the strong activating domain of the herpesvirus protein VP16 onto its C terminus. In response to estrogen, Gal-ER-VP16 induced the Gal4-responsive promoter at least 100-fold in transiently transfected NI...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K S ChristophersonP J Godowski
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G Superti-FurgaM Busslinger
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D M OrnitzP Leder
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S B BaimT Shenk
Jul 1, 1989·Genes & Development·N LevittN J Proudfoot
Feb 10, 1989·Cell·M Beato
Jan 29, 1988·Cell·H Kakidani, M Ptashne
Oct 6, 1988·Nature·I SadowskiM Ptashne
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F M WurmR E Kingston
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N E HynesB Groner

❮ Previous
Next ❯

Citations

Jan 1, 1996·Cytotechnology·T LittlewoodG Evan
Jul 31, 1995·Journal of Biotechnology·G Gross, H Hauser
Apr 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·J Whelan, N Miller
Feb 19, 2004·Brain Research. Molecular Brain Research·Youichi SaitohGuy Boileau
Jun 28, 2005·Trends in Biotechnology·Karuppiah Chockalingam, Huimin Zhao
Feb 8, 2003·Trends in Biotechnology·William M Keyes, Alea A Mills
Jul 27, 2002·Journal of Biotechnology·Wilfried Weber, Martin Fussenegger
Dec 4, 2001·Biochimica Et Biophysica Acta·H Mizuguchi, T Hayakawa
Aug 26, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·B W OzanneG Stapleton
Apr 9, 2013·Analytical Chemistry·Richard H PerryRichard N Zare
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·F ChenC P Austin
Apr 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Karuppiah ChockalingamHuimin Zhao
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·T OsterwalderH Keshishian
Dec 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·M IyerS S Gambhir
Jun 25, 1995·Nucleic Acids Research·W MikulitsE W Müllner
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y WangB W O'Malley
Aug 9, 2002·Molecular and Cellular Biology·Nuray AkyüzLisa Wiesmüller
Mar 1, 2003·Journal of Virology·Vivian RuvoloSankar Swaminathan
Oct 25, 2006·Molecular and Cellular Biology·Thomas HeimbucherThomas Czerny
May 1, 1996·Human Gene Therapy·J P Delort, M R Capecchi
Jul 1, 1995·Somatic Cell and Molecular Genetics·J DhawanH M Blau
Jan 1, 1997·Annual Review of Pharmacology and Toxicology·L N Wei
Apr 8, 1998·Annual Review of Neuroscience·J R Gingrich, J Roder
Jun 8, 2001·Annual Review of Biochemistry·C O PaboR A Grant
Dec 2, 2009·The Journal of Cell Biology·Karmella A Haynes, Pamela A Silver
Apr 19, 2005·Expert Opinion on Biological Therapy·Sergei Zolotukhin
Feb 26, 2016·Journal of Molecular Biology·Kevin C MaTimothy K Lu
Dec 19, 2012·Trends in Biotechnology·Simon Ausländer, Martin Fussenegger
Sep 19, 2009·Current Opinion in Biotechnology·Marcel Tigges, Martin Fussenegger
Oct 16, 2007·Current Opinion in Biotechnology·Wilfried Weber, Martin Fussenegger
Jul 3, 2007·Journal of Biotechnology·David Greber, Martin Fussenegger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.