A Self-Actuated Cellular Protein Delivery Machine

ACS Synthetic Biology
Recep Erdem AhanUrartu Özgür Şafak Şeker

Abstract

Engineered bacterial cells have great promise to solve global problems, yet they are hampered by a lack of convenient strategy for controlled protein release. A well-controlled protein translocation through cellular membranes is essential for cell-based protein delivery. Here we have developed a controlled protein release system by programming a bacterial autotransporter system named Ag43. Ag43 protein is engineered by adding a protease digestion site between its translocation and cargo domains. Once it is displayed on the cell surface, we managed to release the cargo proteins in defined conditions by processing environmental signals. The protein release in terms of time and quantity can be controlled through changing the inducer conditions. We thought that the release system can be adopted for complex genetic circuitries due to its simplicity. We implemented the protein release system to develop a cellular device that is able to release proteins in a sequence response to ordered chemical signals. We envision that development of genetically controlled protein release systems will improve the applications of synthetic organisms in cell based therapies, especially for cases with a need for controlled protein release using the cue...Continue Reading

References

Feb 5, 2000·Nature·T S GardnerJ J Collins
Jun 30, 2000·Protein Expression and Purification·R B Kapust, D S Waugh
May 25, 2002·Science·Călin C GuetStanislas Leibler
Jul 11, 2002·Journal of Bacteriology·Kristian KjaergaardPer Klemm
Nov 18, 2004·Biochimica Et Biophysica Acta·P Delepelaire
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Ian R HendersonDlawer Ala'Aldeen
Jun 1, 2005·Trends in Biotechnology·David S Waugh
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
Dec 5, 1989·Biotechnology and Bioengineering·J W Robbins, K B Taylor
Sep 13, 2008·Annual Review of Microbiology·Marjan W van der Woude, Ian R Henderson
Oct 18, 2008·Science·Maung Nyan Win, Christina D Smolke
Jul 15, 2009·Biotechnology Letters·Ye Ni, Rachel Chen
Sep 17, 2009·Molecular Systems Biology·Daniel M WidmaierChristopher A Voigt
Feb 4, 2010·The Journal of Biological Chemistry·Victoria GirardMichael Mourez
Jul 22, 2010·Journal of Biological Engineering·Robert Sidney CoxMichael B Elowitz
Sep 17, 2010·Nucleic Acids Research·Joseph H DavisRobert T Sauer
Feb 11, 2011·Protein Science : a Publication of the Protein Society·Alexander KotzschMichael Sundström
May 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Julius B LucksAdam P Arkin
Aug 30, 2011·Protein Expression and Purification·David S Waugh
Feb 18, 2012·Nature Reviews. Microbiology·Denisse L LeytonIan R Henderson
Jun 5, 2012·Structure·Gerd PrehnaNatalie C J Strynadka
Sep 29, 2012·The Journal of Biological Chemistry·Balakrishnan RameshNavin Varadarajan
Feb 12, 2013·Nature Biotechnology·Piro SiutiTimothy K Lu
Mar 30, 2013·Science·Jerome BonnetDrew Endy
Oct 17, 2013·Biochimica Et Biophysica Acta·Sabrina ThomasLutz Schmitt
Nov 26, 2013·Biochimica Et Biophysica Acta·Peter van UlsenJoen Luirink
Dec 10, 2013·Nature Chemical Biology·Brynne C StantonChristopher A Voigt
Aug 1, 2014·ACS Synthetic Biology·Jan Claesen, Michael A Fischbach
Dec 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Luke A CliftonJeremy H Lakey
Dec 31, 2014·Nature Methods·Jianyi YangYang Zhang
Mar 31, 2015·Chembiochem : a European Journal of Chemical Biology·Alexander Prokup, Alexander Deiters
Apr 9, 2015·ACS Synthetic Biology·Analise Z ReevesCammie F Lesser
May 29, 2015·Science Translational Medicine·Alexis CourbetJérôme Bonnet
Feb 9, 2016·Nature Chemical Biology·Matthew F CopelandBrian F Pfleger
Jun 15, 2016·Nucleic Acids Research·Jesus Fernandez-Rodriguez, Christopher A Voigt

❮ Previous
Next ❯

Citations

Apr 3, 2021·ACS Synthetic Biology·Cristina Hernández-RollánMorten H H Nørholm
Sep 3, 2020·ACS Synthetic Biology·Pavel DvořákVíctor de Lorenzo
Jan 26, 2022·ACS Synthetic Biology·Anooshay KhanUrartu Ozgur Safak Seker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.