A self-assembled quantum dot probe for detecting beta-lactamase activity

Biochemical and Biophysical Research Communications
Chenjie XuJianghong Rao

Abstract

This communication describes a quantum dot probe that can be activated by a reporter enzyme, beta-lactamase. Our design is based on the principle of fluorescence resonance energy transfer (FRET). A biotinylated beta-lactamase substrate was labeled with a carbocyanine dye, Cy5, and immobilized on the surface of quantum dots through the binding of biotin to streptavidin pre-coated on the quantum dots. In assembling this nanoprobe, we have found that both the distance between substrates and the quantum dot surface, and the density of substrates are important for its function. The fluorescence emission from quantum dots can be efficiently quenched (up to 95%) by Cy5 due to FRET. Our final quantum dot probe, assembled with QD605 and 1:1 mixture of biotin and a Cy5-labeled lactam, can be activated by 32microg/mL of beta-lactamase with 4-fold increase in the fluorescence emission.

References

Apr 20, 1999·Nature Biotechnology·R WeisslederA Bogdanov
Jun 1, 2001·Nature Medicine·C BremerR Weissleder
Feb 19, 2002·Current Opinion in Biotechnology·Warren C W ChanShuming Nie
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tom WehrmanHelen M Blau
Dec 3, 2002·Science·Benoit DubertretAlbert Libchaber
Dec 3, 2002·Nature Biotechnology·Jyoti K JaiswalSanford M Simon
Aug 28, 2003·Nature Materials·Igor L MedintzJ Matthew Mauro
Dec 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sumitaka HasegawaJianghong Rao
Jan 6, 2004·Nature Biotechnology·Paul Alivisatos
Jan 31, 2004·Analytical Chemistry·Ellen R GoldmanHedi Mattoussi
Jun 10, 2004·Journal of the American Chemical Society·Sumitaka HasegawaJianghong Rao
Jul 20, 2004·Nature Biotechnology·Xiaohu GaoShuming Nie
Mar 24, 2005·Journal of the American Chemical Society·Bengang XingJianghong Rao
May 5, 2005·Journal of the American Chemical Society·Ellen R GoldmanHedi Mattoussi
Jun 2, 2005·Nature Materials·Igor L MedintzHedi Mattoussi
Jul 26, 2005·Biochemical and Biophysical Research Communications·Emmanuel ChangJennifer L West
Oct 14, 2005·Journal of the American Chemical Society·Wenzhong GaoJianghong Rao
Feb 28, 2006·Nature Biotechnology·Min-Kyung SoJianghong Rao

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Citations

Dec 4, 2013·Annals of Biomedical Engineering·Sheng TongGang Bao
Sep 29, 2009·Journal of Fluorescence·Junfeng JiangLinli Chen
Jun 1, 2013·Journal of Fluorescence·Shan HuangBaoqing Hu
Oct 31, 2012·Science China. Life Sciences·Sheng TongGang Bao
Feb 27, 2009·Journal of the American Chemical Society·Kelly BoenemanIgor L Medintz
Nov 26, 2008·Chemical Communications : Chem Comm·Shan HuangXiao Niu Peng
Dec 17, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiuling WangYuanhua Meng
Jun 9, 2015·Sensors·Armen ShamirianPreston T Snee
Aug 21, 2009·Expert Opinion on Drug Delivery·James B DelehantyIgor L Medintz
Jun 10, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Tiliang ZhangBin Liu
Jul 16, 2008·Biochemical and Biophysical Research Communications·Min-kyung SoJianghong Rao
Jan 20, 2007·Current Opinion in Biotechnology·Jianghong RaoHequan Yao
Jan 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Hyeong-Gon KangJeeseong Hwang
Mar 24, 2009·IEEE Transactions on Nanobioscience·Yun XingJianghong Rao
Jun 28, 2016·Sensors·Daniel M Jones, Sergi Padilla-Parra
Dec 15, 2006·Biopolymers·Min Zhou, Indraneel Ghosh
Sep 11, 2009·Luminescence : the Journal of Biological and Chemical Luminescence·Lei WangYouqiu He
Jan 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Yi Lu, Juewen Liu
May 10, 2011·Chemical Society Reviews·Andrew RazgulinJianghong Rao
Dec 15, 2015·Chembiochem : a European Journal of Chemical Biology·Young-Pil Kim, Hak-Sung Kim
Dec 17, 2008·Physical Chemistry Chemical Physics : PCCP·Igor L Medintz, Hedi Mattoussi
Mar 11, 2011·Angewandte Chemie·James E GhadialiMolly M Stevens
Apr 10, 2019·Journal of Fluorescence·Sibel BüyüktiryakiArzu Ersöz
Jun 6, 2019·Biosensors·Shashi BhuckoryAllison M Dennis
Jan 30, 2013·Biomaterials Science·Mischa ZelzerRein V Ulijn
Aug 20, 2020·Chemical Reviews·Hongjian HeBing Xu
May 29, 2007·Nano Letters·Igor L MedintzHedi Mattoussi

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