A Self-Assembling Ferritin Nanoplatform for Designing Classical Swine Fever Vaccine: Elicitation of Potent Neutralizing Antibody.

Vaccines
Zekai ZhaoPing Qian

Abstract

Protein-based self-assembling nanoplatforms exhibit superior immunogenicity compared with soluble antigens. Here, we present a comprehensive vaccine strategy for displaying classical swine fever virus (CSFV) E2 glycoprotein on the surface of ferritin (fe) nanocages. An E2-specific blocking antibody assay showed that the blocking rates in pE2-fe/Gel02 (84.3%) and a half-dose cohort of E2-fe/Gel02 (81.9%) were significantly higher (p < 0.05) than that in a ferritin-free cohort of pE2/Gel02 (62.7%) at 21 days post immunization (dpi) in vivo. Furthermore, quantitation of neutralizing potency revealed that a highly significant difference (p < 0.001) was observed between the pE2-fe/Gel02 cohort (1:32, equivalent to live-attenuated strain C at 1:32) and the pE2/Gel02 cohort (1:4) at 21 dpi. Moreover, the innate immune cytokines of IL-4 and IFN-γ activated by the half-dose (20 μg) cohort of E2-fe/Gel02 were equivalent to those elicited by the full dose (40 μg) of purified E2 in the pE2/Gel02 cohort at most time points. In conclusion, we successfully obtained an antigen-displaying E2-ferritin nanoplatform and confirmed high ferritin-assisted humoral and cellular immunities. Our results provided a novel paradigm of self-assembling nanova...Continue Reading

References

Apr 19, 2012·Transboundary and Emerging Diseases·L LuoJ Pasick
Nov 23, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Jae-A HanSebyung Kang
Feb 11, 2016·Computational and Structural Biotechnology Journal·Jacinto López-SagasetaMatthew J Bottomley
Sep 10, 2018·Molecular Therapy. Nucleic Acids·Pavlos C EnglezouKenneth C McCullough
Sep 9, 2020·Virus Research·Llilianne GangesNicolas Ruggli
Nov 6, 2020·Biochemical Pharmacology·Michael CartyAndrew G Bowie

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Methods Mentioned

BETA
Transfection
PCR
confocal microscopy
ELISA

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