A Self-Assembling Two-Dimensional Protein Array is a Versatile Platform for the Assembly of Multicomponent Nanostructures

Biotechnology Journal
Alexander ThomasFrançois Baneyx

Abstract

Rationally designed two-dimensional (2D) arrays that support the assembly of nanoscale components are of interest for catalysis, sensing, and biomedical applications. The computational redesign of a protein called TTM that undergoes calcium-induced self-assembly into nanostructured lattices capable of growing to dozens of micrometers are previously reported. The work demonstrates here that the N- and C-termini of the constituent monomers are solvent-accessible and that they can be modified with a hexahistidine extension, a gold-binding peptide, or a biotinylation tag to decorate nickel-nitriloacetic acid beads with self-assembled protein islands, conjugate gold nanoparticles to planar arrays, or control the immobilization density of avidin molecules onto 2D lattices through co-polymerization of biotinylated and wild type TTM monomers. These results showcase the potential of TTM as a versatile 2D scaffold for the fabrication of hierarchical structures comprising a broad range of nanoscale elements.

References

Oct 1, 1986·Journal of Ultrastructure and Molecular Structure Research·P MessnerU B Sleytr
Apr 22, 1999·Protein Science : a Publication of the Protein Society·D BeckettP J Schatz
Mar 6, 2003·Nature Materials·Rajesh R NaikMorley O Stone
Sep 3, 2003·Nature Materials·Mehmet SarikayaFrançois Baneyx
Oct 2, 2003·Nature Biotechnology·Shuguang Zhang
Mar 1, 2007·FEMS Microbiology Letters·Uwe B SleytrEva M Egelseer
Jul 10, 2007·Current Opinion in Biotechnology·François Baneyx, Daniel T Schwartz
Jan 16, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Atanu SenguptaFrançois Baneyx
Nov 1, 2008·Chemical Reviews·Matthew B DickersonRajesh R Naik
Aug 5, 2010·Journal of Structural Biology·Anding HuangRolf Boelens
Aug 2, 2011·Nature Nanotechnology·John C SinclairMartin E M Noble
Feb 22, 2012·Science·T AidaS I Stupp
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jul 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Seong-Ho ShinJames J De Yoreo
Mar 21, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Brandon L CoyleFrançois Baneyx
May 23, 2013·Nature Chemistry·John W Colson, William R Dichtel
Jan 28, 2014·Current Opinion in Chemical Biology·John C Sinclair
Feb 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey D BrodinF Akif Tezcan
Apr 30, 2014·Biotechnology and Bioengineering·Brandon L Coyle, François Baneyx
May 17, 2014·Current Opinion in Biotechnology·François Baneyx, James F Matthaei
May 27, 2014·Accounts of Chemical Research·Wade W Grabow, Luc Jaeger
Jun 5, 2014·Angewandte Chemie·Ali Aghebat RafatFriedrich C Simmel
Oct 7, 2014·Nature Nanotechnology·F H L KoppensM Polini
Aug 28, 2015·Nature Communications·Yuki SuzukiHiroshi Sugiyama
Nov 7, 2015·ACS Nano·Ganesh R BhimanapatiJoshua A Robinson
Aug 5, 2016·Chemical Society Reviews·Juan WangXuehai Yan
Sep 16, 2016·Nature·Po-Ssu HuangDavid Baker
Jan 25, 2018·Advanced Materials·Sami NummelinVeikko Linko

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Citations

Dec 12, 2018·Biotechnology Journal·William E BentleyTakeshi Gotoh
Jul 25, 2020·Biochemistry·Rohit H SubramanianF Akif Tezcan
Oct 22, 2019·Chemistry of Materials : a Publication of the American Chemical Society·Babatunde O OkesolaAlvaro Mata
Aug 19, 2021·Chemical Reviews·Jie ZhuF Akif Tezcan

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