A Self-Contained Chemiluminescent Lateral Flow Assay for Point-of-Care Testing

Analytical Chemistry
Jinqi DengXingyu Jiang

Abstract

Immunoassays whose readouts rely on chemiluminescence are increasingly useful for a broad range of analytical applications, but they are rarely made into point-of-care (POC) format because of the complex reagents required (some reagents have to be stored in low temperatures, and some reagents have to be freshly made right before the assay). This study reports a self-contained chemiluminescent lateral flow assay (CLFA), which prestores all necessary reagents. This CLFA contains three parts: the normal lateral flow assay (LFA) strip, the chemiluminescence substrate pad, and the polycarbonate (PC) holder. On the LFA strip, we simultaneously labeled horseradish peroxidase (HRP) and antibody on the gold nanoparticles (AuNPs) for the conjugate pad. For the substrate pad, we used sodium perborate as the oxidant and lyophilized the chemiluminescence substrate on the glass fiber, which allows long-term storage. After the transfer of substrate from the substrate pad to the nitrocellulose (NC) membrane, we captured the chemiluminescence signal for the quantification of the targets. The HRP on the AuNPs can amplify the chemiluminescence signal efficiently. We used this CLFA system to detect both macromolecules and small molecules successfu...Continue Reading

References

Nov 16, 2010·Chemical Communications : Chem Comm·Xuemei LiYingshu Guo
Jun 7, 2013·Advanced Materials·Yi ZhangXingyu Jiang
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Jan 10, 2017·ACS Applied Materials & Interfaces·Zhiqin LiangLei Zhou
Mar 4, 2017·Analytical Chemistry·Wenjing KangIan Papautsky
Jul 21, 2017·Biosensors & Bioelectronics·Mohammad Zarei
Nov 7, 2017·Biosensors & Bioelectronics·Pattarachaya PreechakasedkitOrawon Chailapakul
Dec 8, 2017·Science Advances·Mingzhu YangXingyu Jiang
Nov 4, 2016·Light, Science & Applications·Alina KarabchevskyAlexey V Kavokin

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Citations

Sep 24, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yihong ZhongQuan Cheng
May 20, 2020·Chemistry : a European Journal·Hui OuyangZhifeng Fu
Dec 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yue ZhangYanfei Shen
Apr 30, 2021·Analytical and Bioanalytical Chemistry·Antonia Perju, Nongnoot Wongkaew
May 4, 2021·Talanta·Elham SheikhzadehMohammed Zourob
Jan 15, 2020·Chemical Science·Jiangjiang ZhangXingyu Jiang
Jun 3, 2021·Sensors·Donato CalabriaMara Mirasoli
Jul 3, 2021·Sensors·Maria Maddalena CalabrettaElisa Michelini
Jan 7, 2020·ACS Applied Materials & Interfaces·Qingyun LiuFuyou Li

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