PMID: 19915746Nov 17, 2009Paper

A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line

Biological Research
Evelyn Aranda, Gareth I Owen

Abstract

Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in matrigel. This valid and simple method has the drawbacks that each cell population is distinct and the constraint of obtaining primary source material. Herein we utilize the established EA.hy926 endothelial cell line as our model for in vitro angiogenesis and present a novel formula to quantify endothelial cell remodeling to identify pro- and anti-angiogenic agents. Furthermore, our technique details the procedures to identify and quantify compounds that have the capacity to generate pro- or anti-angiogenic factors when given to non-endothelial cells, which we define herein as angiogenic potential. In conclusion, we propose a novel formula that we are confident accurately reflects the degree of in vitro angiogenesis allowing the quantification of prospective angiogenic compounds.

Citations

Aug 19, 2011·Clinical Oral Investigations·Shuei-Kuen TsengJiiang-Huei Jeng
Apr 26, 2014·Hormones & Cancer·Mauricio P PintoGareth I Owen
Nov 13, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Marisol QuezadaMiguel L Allende
Sep 9, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Alejandro CuevasLuis A Salazar
Jun 2, 2011·International Journal of Cancer. Journal International Du Cancer·Simona TavernaRiccardo Alessandro
Mar 20, 2016·Fertility and Sterility·Soledad HenríquezLuigi Devoto
Dec 2, 2011·Journal of Cellular and Molecular Medicine·Anne Q ReuwerKeren S Borensztajn
Jul 27, 2012·British Journal of Pharmacology·Bo WuRui Wang
Jan 18, 2011·Gynecologic Oncology·Verónica TapiaCarmen Romero
May 23, 2013·International Journal of Radiation Biology·Charlotte RomboutsSarah Baatout
Jun 19, 2013·Biochemical and Biophysical Research Communications·Jia JianChia Soo
Sep 6, 2014·Frontiers in Pharmacology·Pablo J SáezMarcelo Farías-Jofré
Sep 28, 2016·Oxidative Medicine and Cellular Longevity·Javier PalaciosPedro Buc Calderon
Jan 26, 2013·International Journal of Molecular Sciences·Maria Letizia TrincavelliClaudia Martini
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Mar 13, 2014·Journal of Cellular Physiology·Soledad LangeGareth I Owen
Aug 13, 2013·Experimental and Therapeutic Medicine·Wei Min HuangXiao Hong Wang
Dec 15, 2010·American Journal of Physiology. Cell Physiology·Nitza LahatMichal A Rahat
Jul 6, 2017·International Journal of Molecular Medicine·Jia Yu HeWei Min Huang
Jan 15, 2015·Plastic and Reconstructive Surgery. Global Open·Zhong ZhengChia Soo

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