A sensitive amperometric immunosensor for alpha-fetoprotein based on carbon nanotube/DNA/Thi/nano-Au modified glassy carbon electrode

Colloids and Surfaces. B, Biointerfaces
Xiao-Qi RanXiao-Qing Qian

Abstract

A novel amperometric immunosensor for the determination of alpha-fetoprotein (AFP) was constructed using films of multi-wall carbon nanotubes/DNA/thionine/gold nanoparticles (nano-Au). Firstly, multiwall carbon nanotubes (MWCNT) dispersed in poly(diallydimethlammonium chloride) (PDDA) were immobilized on the nano-Au film which was electrochemically deposited on the surface of glassy carbon electrode. Then a negatively charged DNA film was absorbed on the positively charged PDDA. Subsequently, thionine was attached to the electrode via the electrostatic interaction between thionine and the DNA. Finally, the nano-Au was retained on the thionine film for immobilization of AFP antibody (anti-AFP). The modification process was characterized by cyclic voltammetry (CV) and scanning electron microscope (SEM). The factors possibly influenced the performance of the proposed immunosensors were studied in detail. Under optimal conditions, the proposed immunosensor exhibited good electrochemical behavior to AFP in a two concentration ranges: 0.01-10.0 and 10.0-200.0 ng/mL with a relatively low detection limit of 0.04 ng/mL at three times the background noise. Moreover, the selectivity, repeatability and stability of the proposed immunosenso...Continue Reading

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Citations

Feb 3, 2012·Chemical Reviews·Krishnendu SahaVincent M Rotello
Dec 17, 2011·International Journal of Molecular Sciences·Ning GanLei Zheng
Oct 21, 2011·Journal of Automated Methods & Management in Chemistry·Ning GanLei Zheng
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Jul 17, 2018·Scanning·Kazuo Umemura, Shizuma Sato
Jan 14, 2021·Biomedical Physics & Engineering Express·Saloni TandonBalasubramanian Kandasubramanian
Apr 4, 2019·Analytical Chemistry·Ru ZhangKun Qian

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