Abstract
A novel amperometric immunosensor for the determination of alpha-fetoprotein (AFP) was constructed using films of multi-wall carbon nanotubes/DNA/thionine/gold nanoparticles (nano-Au). Firstly, multiwall carbon nanotubes (MWCNT) dispersed in poly(diallydimethlammonium chloride) (PDDA) were immobilized on the nano-Au film which was electrochemically deposited on the surface of glassy carbon electrode. Then a negatively charged DNA film was absorbed on the positively charged PDDA. Subsequently, thionine was attached to the electrode via the electrostatic interaction between thionine and the DNA. Finally, the nano-Au was retained on the thionine film for immobilization of AFP antibody (anti-AFP). The modification process was characterized by cyclic voltammetry (CV) and scanning electron microscope (SEM). The factors possibly influenced the performance of the proposed immunosensors were studied in detail. Under optimal conditions, the proposed immunosensor exhibited good electrochemical behavior to AFP in a two concentration ranges: 0.01-10.0 and 10.0-200.0 ng/mL with a relatively low detection limit of 0.04 ng/mL at three times the background noise. Moreover, the selectivity, repeatability and stability of the proposed immunosenso...Continue Reading
References
Feb 28, 2001·Fresenius' Journal of Analytical Chemistry·A WarsinkeF W Scheller
Mar 20, 2003·Journal of Agricultural and Food Chemistry·Serena LaschiMarco Mascini
Jun 15, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Pingang He, Mekki Bayachou
Feb 24, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Yuanhua DingRong Guo
Feb 11, 2009·Talanta·Xia YangHuilan Su
Citations
Feb 3, 2012·Chemical Reviews·Krishnendu SahaVincent M Rotello
Dec 17, 2011·International Journal of Molecular Sciences·Ning GanLei Zheng
Feb 28, 2013·Sensors·Xia SunJinmei Gao
Oct 21, 2011·Journal of Automated Methods & Management in Chemistry·Ning GanLei Zheng
Nov 21, 2012·Bioelectrochemistry·Ke-Jing HuangYan-Ming Liu
May 13, 2011·Colloids and Surfaces. B, Biointerfaces·Lingsong LuGuoming Xie
Mar 1, 2011·Colloids and Surfaces. B, Biointerfaces·Haiyan WangLun Wang
Aug 2, 2015·Biosensors & Bioelectronics·Na LiQin Wei
Aug 30, 2011·Talanta·Xia GaoXianfu Lin
Feb 7, 2012·Biosensors & Bioelectronics·Lingsong LuGuoming Xie
Nov 24, 2011·The Analyst·Lina ZouBaoxian Ye
Sep 13, 2011·Analytical Chemistry·Marcos EguílazJosé M Pingarrón
Dec 19, 2012·Analytical Chemistry·Ali A EnsafiBehzad Rezaei
Jul 17, 2018·Scanning·Kazuo Umemura, Shizuma Sato
Aug 21, 2019·Nanotechnology·Feng LongPenghui Li
Nov 7, 2019·Scientific Reports·Belen BeginesRafael Prado-Gotor
Jan 14, 2021·Biomedical Physics & Engineering Express·Saloni TandonBalasubramanian Kandasubramanian
Oct 4, 2019·Biosensors & Bioelectronics·Zhiyi YuanChao Yu
Apr 7, 2021·Chemosphere·Zeynab KhorablouHassan Karimi-Maleh
Apr 4, 2019·Analytical Chemistry·Ru ZhangKun Qian
Nov 23, 2021·ACS Biomaterials Science & Engineering·Koushik DuttaDipankar Chattopadhyay