A sensitive and high-throughput assay to detect low-abundance proteins in serum

Nature Medicine
Hongtao ZhangM I Greene

Abstract

The ability to detect antigens immunologically is limited by the affinity of the antibodies and the amount of antigens. We have now succeeded in creating a modular, facile amplification system, termed fluorescent amplification catalyzed by T7 polymerase technique (FACTT). Such a system can detect protein targets specifically at subfemtomolar levels ( approximately 0.08 fM). We describe here the detection of Her2 (also known as Neu) from rodent and human sera. FACTT is adaptable to high-throughput screening and automation and provides a practical method to enhance current ELISAs in medical practice.

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Citations

Feb 1, 2012·Analytical and Bioanalytical Chemistry·Berta Esteban-Fernández de ÁvilaJosé M Pingarrón
Feb 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Stephanie A KazaneVaughn V Smider
Oct 31, 2006·Omics : a Journal of Integrative Biology·Adriana Aguilar-MahechaMark Basik
Aug 24, 2012·Journal of the Royal Society, Interface·James EberwineJunhyong Kim
Oct 17, 2007·Annual Review of Medicine·James R Heath, Mark E Davis
Aug 3, 2007·The Journal of Clinical Investigation·Hongtao ZhangMark I Greene
Jun 16, 2011·International Journal of Molecular Sciences·Hongtao ZhangXiaowei Xu
Oct 4, 2006·Expert Opinion on Drug Metabolism & Toxicology·Vishal S Vaidya, Joseph V Bonventre
Apr 24, 2012·Biochimica Et Biophysica Acta·Lian LamHongtao Zhang
Sep 21, 2010·Seminars in Cell & Developmental Biology·Zheng CaiMark I Greene
Feb 2, 2008·Toxicology·Fitz B Collings, Vishal S Vaidya
Jun 13, 2015·SpringerPlus·Julia TchouHongtao Zhang
Nov 10, 2015·Chemical Reviews·Yongxi ZhaoChunhai Fan
Aug 24, 2013·Clinical & Developmental Immunology·Anja LukanVladka Curin Šerbec
May 16, 2014·Journal of Gastroenterology·Hideaki Shimada
Jul 28, 2016·Food Chemistry·Víctor Ruiz-Valdepeñas MontielJosé M Pingarrón
Jan 1, 2012·Pharmaceuticals·Ramachandran Murali, Mark I Greene
Jul 25, 2007·The Analyst·Hongquan ZhangX Chris Le
Jan 12, 2007·Molecular BioSystems·Uwe R Müller
May 30, 2008·Clinical Chemistry·Michael G KattahImelda Balboni
Nov 3, 2006·Clinical and Vaccine Immunology : CVI·Binggong ChangMan-Sun Sy
Mar 30, 2011·Experimental Biology and Medicine·Ping WangSrinand Sreevatsan

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