A sensitive and practical RP-HPLC-FLD for determination of the low neuroactive amino acid levels in body fluids and its application in depression

Neuroscience Letters
Juan-Li WuAi-Min Bao

Abstract

Ion-exchange high performance liquid chromatography (HPLC) generally fails as a method to determine low levels of free amino acids (AAs) in body fluids. Here we present a modified reversed-phase HPLC (RP-HPLC) protocol for the determination of AAs in body fluids and its application in mood disorder patients. We improved a previous research protocol by modifying i) sample preparation, including deproteination, ii) derivitization, including derivating agent and condition, and iii) sample separation, which is mainly determined by the pH value, the components and the additives of the mobile phases. The combination of these modifications, together with fluorescence detection (FLD), allows sensitive and practical determination of free AA levels in body fluids of depressive patients. This protocol was validated by determining the postmortem cerebrospinal fluid (CSF) glutamic acid (Glu) and γ-aminobutyric acid (GABA) levels of 8 major depressive disorder (MDD) patients, 9 bipolar disorder (BD) patients, and 19 well-matched controls, while also testing the plasma and CSF AA levels of living MDD patients. CSF Glu and GABA levels were both significantly decreased in MDD but not in BD patients. The data indicate that this RP-HPLC-FLD proto...Continue Reading

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Citations

Oct 12, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiaomin LiMakoto Tsunoda
Nov 3, 2017·Biochemical Genetics·Muhammad WasimHina Ayesha
Jan 3, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xue-Jiao ZhaoZhao-Ying Liu
Nov 30, 2020·Brain, Behavior, and Immunity·Qiuyuan FangLing Shan
Sep 26, 2021·Metabolomics : Official Journal of the Metabolomic Society·Rezvan MarzabaniMohieddin Jafari

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