PMID: 2494264Mar 10, 1989Paper

A sensitive double-diffusion microassay suitable for the detection of idiotype-antiidiotype precipitates

Journal of Immunological Methods
D RochuJ M Fine

Abstract

Using agarose gel coated on GelBond film sheets and using Coomassie blue stain followed by silver stain, a sensitive double-diffusion microassay has been developed for detecting small amounts of precipitate forming during idiotype-antiidiotype reactions. The sensitivity of the method is 10-100 times greater than classical immunodiffusion tests. Other advantages include the need for minimal amounts of unconcentrated sample, the absence of radioactive or toxic substrates, no interference due to a second or third antibody coat such as are used in immunoenzymatic techniques, and the possibility of a direct evaluation of qualitative data such as identity, cross-reactivity or non-identity. As little as 40 ng antibody could be detected, corresponding to an antibody concentration of 8 micrograms/ml, making the microassay useful for rapid screening of idiotype-antiidiotype precipitates during routine analysis of hybridoma supernatants.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P A Cazenave
Dec 1, 1987·Revue française de transfusion et immuno-hématologie·C Salmon
Jan 1, 1980·International Archives of Allergy and Applied Immunology·R M Barnes
Apr 15, 1983·Analytical Biochemistry·E W Willoughby, A Lambert
Jul 1, 1960·The Journal of Clinical Investigation·R S YALOW, S A BERSON

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Citations

Jul 9, 2013·Biologicals : Journal of the International Association of Biological Standardization·Javid ArifPervez Akhtar
Apr 25, 2008·Current Protocols in Immunology·P Hornbeck
Feb 6, 2017·Current Protocols in Immunology·Peter Hornbeck

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