A sensitive electrophoretic method for the quantification of myosin heavy chain isoforms in horse skeletal muscle: histochemical and immunocytochemical verifications

Electrophoresis
J L RiveroV R Edgerton

Abstract

In adult horses, three myosin heavy chain (MyHC) isoforms can be identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunohistochemistry using specific anti-MyHC monoclonal antibodies. This report studies the suitability of a consistent SDS-PAGE technique for quantifying MyHC profiles in homogenized cryostate sections of equine gluteus medius muscle biopsies (n = 18). The method used (previously described by R. J. Talmadge and R. R. Roy; J. Appl. Physiol. 1993, 75, 2337-2340) resolved MyHCs in three bands: I, IIB or IIX, and IIA from the fastest to the slowest migration band. The success rate of the protocol for yielding three well-differentiated MyHC bands was 100% and a subsequent quantification by densitometry for each MyHC isoform was obtained in all 18 muscle biopsies. The results obtained with this electrophoretic method were compared with routine myofibrillar adenosine triphosphatase histochemistry and immunohistochemistry using specific anti-MyHC monoclonal antibodies. The percent composition of the three electrophoretically separated MyHC isoforms (I, IIA and IIB or IIX) showed strong positive correlation with percentages of the area occupied in the biopsies by the three major fiber ...Continue Reading

References

Mar 1, 1978·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·T E Sullivan, R B Armstrong
Nov 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A A SosnickiD O Goble
Jun 1, 1989·Journal of Muscle Research and Cell Motility·S SchiaffinoT Lømo
Jun 1, 1989·Journal of the Neurological Sciences·M Ecob-PrinceW Brown
Jun 1, 1986·Journal of Applied Physiology·D B ThomasonR E Herrick
Oct 1, 1970·Archives of Neurology·M H Brooke, K K Kaiser
Feb 1, 1995·Journal of Muscle Research and Cell Motility·S EnnionG Goldspink
Jan 1, 1994·European Journal of Applied Physiology and Occupational Physiology·A C FryR S Staron
Nov 1, 1993·Journal of Applied Physiology·R J Talmadge, R R Roy
Jan 1, 1995·Reproduction, Nutrition, Development·E BarreyJ Robelin
Apr 1, 1996·Physiological Reviews·S Schiaffino, C Reggiani
Apr 1, 1996·The Anatomical Record·A L SerranoJ W Hermanson
Dec 1, 1987·Trends in Ecology & Evolution·C R Smith, R R Hessler

❮ Previous
Next ❯

Citations

Feb 1, 2011·Histochemistry and Cell Biology·Stefan Hübner, Athina Efthymiadis
Mar 1, 1997·Brain Research. Molecular Brain Research·C E BlancoP Micevych
Nov 19, 2003·The Veterinary Journal·Robert A LehnhardMatthew D Beekley
Oct 31, 2002·Equine Veterinary Journal. Supplement·J L L RiveroJ Harmeyer
Aug 3, 2002·American Journal of Respiratory and Critical Care Medicine·Heather K VincentScott K Powers
Nov 8, 2008·American Journal of Respiratory Cell and Molecular Biology·Harry R GoskerAnnemie M W J Schols
Nov 17, 2012·The Journal of Experimental Biology·Tertius A Kohn, Timothy D Noakes
Mar 3, 2007·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Erik P RaderJohn A Faulkner
Jan 29, 2014·Acta Veterinaria Scandinavica·Heli K HyytiäinenCatherine M McGowan
May 4, 2010·Research in Veterinary Science·A K MykkänenB Essén-Gustavsson
Jul 17, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Tertius A KohnKathryn H Myburgh
Apr 4, 2007·Equine Veterinary Journal. Supplement·J L RiveroA Lindner
Dec 1, 1996·The Annals of Thoracic Surgery·G A ThomasL W Stephenson
Oct 4, 2014·Meat Science·Rui LiuGuang-hong Zhou
Aug 7, 2007·BMC Cancer·Jeffrey S OtisJay H Williams
Jan 14, 2016·Korean Journal for Food Science of Animal Resources·Ho-Kyoung KimYoun-Chul Ryu
Feb 2, 2010·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jean-François DesaphyDiana Conte Camerino
Jan 30, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Helga GuderleyTheodore Garland
Jul 19, 2005·Experimental Neurology·Marie-Agnès Giroux-MetgesMaxime Gioux
Jul 2, 2008·The American Journal of Cardiology·Maria Antonietta PellegrinoGiuseppe D'Antona
Feb 16, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Tertius Abraham KohnTimothy David Noakes
May 27, 2003·Journal of Dental Research·J A M Korfage, T M G J Van Eijden
Oct 4, 2006·The Journal of Physiological Sciences : JPS·Tertius Abraham Kohn, Kathryn Helen Myburgh
Oct 29, 2009·Physiological Genomics·Suzanne S EiversEmmeline W Hill
Jan 27, 2007·Journal of Applied Physiology·José-Luis L RiveroArno Lindner
Feb 5, 2000·Equine Veterinary Journal. Supplement·J L Rivero, A L Serrano

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.