PMID: 6169764Jan 1, 1981Paper

A sensitive enzyme-linked immunosorbent assay for the quantitation of antigen-specific murine immunoglobulin E

Journal of Immunological Methods
P N Hill, F T Liu

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the quantitation of 2,4-dinitrophenyl (DNP)-specific murine immunoglobulin (Ig) E is described. The assay uses beta-galactosidase, which is conjugated to goat anti-rabbit gamma-globulin (GARG) via a method using a mild heterobifunctional cross-linking reagent, m-maleimidobenzoyl-N-hydroxy-succinimide ester (MBS). The assay has a sensitivity for detection of about 200 pg/ml of anti-DNP IgE. Analyses of murine serum samples using this ELISA correlate well with those obtained using the passive cutaneous anaphylaxis (PCA) reaction in rats. With the use of automated 96-well reader, data acquisition is rapid and, therefore, this ELISA is ideal for analyses of large numbers of samples. The assay can be easily modified for the measurement of other Ig classes and of IgE of other antigen specificities.

References

Nov 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·A H Schuurs, B K Van Weemen
Mar 1, 1979·Scandinavian Journal of Immunology·T KarlssonH Bennich
Mar 1, 1979·Scandinavian Journal of Immunology·T KarlssonH Bennich
Jan 1, 1978·Clinical Allergy·K AlankoS Ojanen
Jan 1, 1976·Journal of Biochemistry·T Kitagawa, T Aikawa
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN

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Citations

Dec 6, 2017·Journal of Immunology Research·Jonathan LaiñoStefan Schülke

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