PMID: 6990557Jan 1, 1980Paper

A sensitive enzymeimmunoassay with a fluorimetric end-point for the determination of testosterone in female plasma and saliva

Steroids
A TurkesD Riad-Fahmy

Abstract

A fluorimetric enzymeimmunoassay has been developed having the sensitivity (500 fg/assay tube) required for determining testosterone concentrations in female plasma and saliva samples. The assay featured a solid-phase antiserum raised against an 11 alpha-hydroxytestosterone-11-hemisuccinate bovine serum albumin conjugate, an 11 alpha-hydroxytestosterone-11-hemisuccinate horseradish peroxidase conjugate as the "enzyme label", and p-hydroxyphenylacetic acid as the substrate for the development of fluorescence. Specificity was ensured by "extracting" testosterone from samples with a solid-phase anti testosterone-3-/0-carboxymethyl/-oxime serum. The assay was shown to satisfy accepted validation criteria providing results in good agreement with routine radioimmunoassay procedures in both plasma (r greater than 0.98, n=28) and saliva (r greater than 0.99, n=28). In saliva samples collected at 2 hourly intervals by normal healthy women (n=5) testosterone concentrations showed a well defined circadian rhythm: the mean testosterone concentration in early morning samples (174 pmol/litre) fell by 83% in late evening collections. In healthy female volunteers (n=7), mean daily throughout one complete cycle ranged from 50 to 218 pmol/litre....Continue Reading

References

Jul 1, 1977·Steroids·K TateishiC Hayashi
Jul 1, 1977·Steroids·K M RajkowskiM F Jayle

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Citations

Jan 1, 1990·Biomedical Chromatography : BMC·K Robards, P Towers
Mar 30, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·S G JohnsonJ M Burrin
Sep 20, 1993·Annals of the New York Academy of Sciences·G F Read
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Nov 30, 2010·Journal of Immunoassay & Immunochemistry·Tulsidas G ShrivastavDinesh Kumar
Jun 29, 2012·Journal of Immunoassay & Immunochemistry·Tulsidas G ShrivastavDinesh Kumar
Jan 1, 1991·The International Journal of Neuroscience·M Hassler
Mar 1, 1986·Annals of Clinical Biochemistry·A A IsmailM Wheeler

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