PMID: 3764344Oct 1, 1986Paper

A sensitive method for the detection of specific antibody production in different isotypes from single lamina propria plasma cells

Scandinavian Journal of Immunology
N Lycke

Abstract

A sensitive and reproducible method for the detection of specific antibody production (or total immunoglobulin secretion) at the single cell level from isolated lamina propria lymphocytes was developed. The cells were prepared from mouse intestinal mucosa by enzyme extraction with collagenase, and antibody secretion was demonstrated with a solid phase enzyme-linked immunospot (ELISPOT) assay. Oral immunizations with cholera toxin or keyhole limpet haemocyanin to mice gave high numbers of highly antigen-specific spot-forming cells (SFC) among isolated lamina propria lymphocytes. Spots were shown to result from active synthesis of immunoglobulin in vitro. The variation in SFC numbers between individual animals after a given protocol of oral immunizations was found to be 25% and between equal groups analysed on different occasions, 12%. Kinetics of primary as well as secondary immune responses after oral immunizations with cholera toxin were easily monitored. A single dose of cholera toxin gave rise to 230 antitoxin SFC/10(7) isolated lamina propria lymphocytes. Each additional dose stimulated to increasing numbers of specific SFC with roughly 7000 antitoxin SFC/10(7) cells after five immunizations. Monitoring of day-by-day respon...Continue Reading

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Citations

Nov 5, 1987·Journal of Immunological Methods·P J Van der Heijden, W Stok
Nov 16, 1995·Journal of Immunological Methods·H AsanumaS Tamura
Mar 28, 1997·Journal of Immunological Methods·H AsanumaS Tamura
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A BandeiraS Tonegawa
Feb 11, 2014·Journal of Immunological Methods·Andrew W GoodyearElizabeth P Ryan
Mar 1, 1994·JPEN. Journal of Parenteral and Enteral Nutrition·H SerizawaM Tsuchiya
Feb 3, 2015·Current Protocols in Immunology·Nils Lycke, Richard Coico
Sep 1, 1993·European Journal of Immunology·E Hörnquist, N Lycke
Oct 12, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roberto Di NiroLudvig M Sollid
Mar 10, 2001·The Journal of Nutrition·K Thoreux, D L Schmucker
Oct 1, 1991·Infection and Immunity·E HörnqvistN Lycke
Aug 1, 1991·Journal of Clinical Microbiology·A A MerchantR D Shaw

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