PMID: 6984053Nov 26, 1982Paper

A sensitive method to measure PHA-induced cytotoxicity to adherent target cells using [3H]uridine as a terminal label

Journal of Immunological Methods
W C GadiotT H The

Abstract

This paper describes a modification of the PHA-induced cytotoxicity test of human peripheral blood lymphocytes against a tumour-derived adherent cell line (HeLa), in which the surviving target cells are labelled with [3H]uridine at the end of the assay. There is a direct correlation between [3H]uridine incorporation and the number of adherent target cells. The test proves to be very sensitive at low effector; target cell ratios. Frozen stored cells can be used in this system, a particular advantage because of the possibility of increasing the reproducibility of the assay by using the same batch of cryopreserved lymphocytes as a reference standard in each experiment. PHA-induced cytotoxicity was mainly found in the T cell enriched fraction.

References

Mar 1, 1977·European Journal of Immunology·M H van OersP T Schellekens
Jan 1, 1978·Journal of Immunological Methods·G Huges-LawT H The
Jan 1, 1976·Journal of Surgical Oncology·N Suciu-FocaK Reemtsma
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen
Jan 1, 1980·Journal of Immunological Methods·C D CalleryS H Golub
Jan 1, 1981·Journal of Immunological Methods·E Piccolella, G Lombardi

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Citations

Jun 1, 1986·Biulleten' eksperimental'noĭ biologii i meditsiny·I V Bogdashin
Jun 1, 1987·Biulleten' eksperimental'noĭ biologii i meditsiny·V V MalaĭtsevV S Zueva

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