A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products.

Toxins
Weiqi ZhangPeiwu Li

Abstract

Sensitive and point-of-care detection of small toxic molecules plays a key role in food safety. Aflatoxin, a typical small toxic molecule, can cause serious healthcare and economic issues, thereby promoting the development of sensitive and point-of-care detection. Although ELISA is one of the official detection methods, it cannot fill the gap between sensitivity and point-of-care application because it requires a large-scale microplate reader. To employ portable readers in food safety, Pt-catalysis has attracted increasing attention due to its portability and reliability. In this study, we developed a sensitive point-of-care aflatoxin detection (POCAD) method via a portable handheld barometer. We synthesized and characterized Au@PtNPs and Au@PtNPs conjugated with a second antibody (Au@PtNPs-IgG). A competitive immunoassay was established based on the homemade monoclonal antibody against aflatoxins. Au@PtNPs-IgG was used to catalyze the production of O2 from H2O2 in a sealed vessel. The pressure of O2 was then recorded by a handheld barometer. The aflatoxin concentration was inversely proportional to the pressure recorded via the barometer reading. After optimization, a limit of detection of 0.03 ng/mL and a linear range from 0....Continue Reading

References

Jun 2, 2009·Analytical and Bioanalytical Chemistry·Gordon S Shephard
Feb 23, 2010·Environmental Health Perspectives·Yan Liu, Felicia Wu
Oct 1, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Thomas W KenslerJohn D Groopman
Oct 24, 2014·Analytical Chemistry·Yishun HuangChaoyong James Yang
Sep 25, 2017·The Science of the Total Environment·Zhenjiang LiuJiawen Luan
Oct 22, 2017·Journal of Hazardous Materials·Chao ZhuAiliang Chen
Mar 3, 2018·The Analyst·Dan LiuChaoyong Yang

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Methods Mentioned

BETA
ELISA
X-ray
transmission
electron diffraction
XRD
Assay
transmission electron microscopy

Software Mentioned

POCAD

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