PMID: 591722Jan 1, 1977

A sensitive radioimmunoassay for a component of mouse casein

Journal of Immunological Methods
J Enami, S Nandi

Abstract

Mouse casein (m.w. 22,000 daltons) has been purified by employing Sephadex G-100 and DEAE-cellulose column chromatographies. A sensitive radioimmunoassay method has been developed by using [125I]-labelled casein and antiserum elicited in rabbits after injection of glutaraldehyde-treated casein. The assay method is capable of detecting as little as 0.1 ng of casein. The use of the present radioimmunoassay method in detecting casein production in cultured mouse mammary explants has also been demonstrated.

References

Apr 1, 1976·The Journal of Experimental Zoology·R L Ceriani
Nov 14, 1975·Science·H N Rose, C M McGrath
Dec 1, 1975·Journal of Immunological Methods·P M TerryM R Banerjee
Sep 1, 1974·Comparative Biochemistry and Physiology. A, Comparative Physiology·M K Feldman
Nov 1, 1974·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J C Hendrick, P Franchimont
Dec 1, 1970·Comparative Biochemistry and Physiology·M K Feldman, R L Ceriani
Aug 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·W G JuergensJ J Elias

Citations

Aug 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H A HahmT Oka
Feb 1, 1984·Journal of Reproductive Immunology·P Weisz-CarringtonM E Lamm
Feb 1, 1991·The Journal of Dairy Research·M SimíckováL Slepicka
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B K Levay-YoungS Nandi
May 1, 1985·The Journal of Cell Biology·J F Levine, F E Stockdale
Jan 14, 2016·Environmental Science & Technology·Shinichi EnamiAgustin J Colussi
Jan 1, 1982·Differentiation; Research in Biological Diversity·D FlynnS Nandi
Aug 1, 1996·Journal of Cellular Physiology·Y K HomS Nandi
Aug 24, 2001·Journal of Dairy Science·M YonedaS Sakai
Mar 1, 1979·Clinical Toxicology

Related Concepts

Metazoa
Casein A
Chromatography, DEAE-Cellulose
Molecular Sieve Chromatography
Dose-Response Relationship, Drug
Immune Sera
Udder
Prolactin
Radioimmunoassay
Culture Techniques

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