PMID: 375481Mar 1, 1979Paper

A sensitive solid phase enzymeimmunoassay for testosterone in plasma and saliva

Steroids
A O TurkesD Riad-Fahmy

Abstract

A sensitive, solid phase enzymeimmunoassay suitable for determining testosterone concentrations in samll aliquots of plasma (20 microliter) and saliva (200 microliter) has been developed. A solid phase antiserum raised against a testosterone-11 alpha-hemisuccinate/bovine serum albumin conjugate was prepared by coupling to cyanogen bromide activated cellulose. The "enzyme label" was a covalently linked testosterone/horseradish peroxidase conjugate. The assay had a lower limit of sensitivity of 4pg/assay tube and satisfied accepted criteria of specificity and precision. Testosterone concentrations determined by enzyme-immunoassay were in excellent agreement not only with a gas liquid chromatography/mass spectrometry procedure (r=0.96, n=12) but also with the radioimmunoassay in routine use (r=0.95, n=12). The EIA can therefore replace RIA in both the small clinical laboratory and high throughput service centres for determining plasma and salivary testosterone concentrations. In normal males salivary testosterone concentrations reflected circulating steroid levels and indicated the possibility of assaying saliva rather than plasma in clinical studies.

Citations

Jan 1, 1999·Indian Journal of Clinical Biochemistry : IJCB·M BanerjeeC Das
Mar 30, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·S G JohnsonJ M Burrin
Jul 1, 1981·Steroids·M J SauerA D Cookson
Jul 1, 1992·Journal of Pharmaceutical and Biomedical Analysis·C J WangC C Lin
Sep 20, 1993·Annals of the New York Academy of Sciences·G F Read
Jan 1, 2011·African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM·Chukwunonso E C C Ejike, Lawrence U S Ezeanyika
Dec 5, 2012·Annals of Medical and Health Sciences Research·Cecc Ejike, Lus Ezeanyika
Oct 4, 2011·Indian Journal of Clinical Biochemistry : IJCB·Bratati SinghPrakash Chandra Mohapatra
Apr 7, 1989·Journal of Chromatography·E H JansenR H van den Berg
Aug 8, 2009·Journal of Immunological Methods·John S Mitchell, Tim E Lowe
Feb 1, 1984·European Journal of Cancer & Clinical Oncology·A TurkesK Griffiths
Nov 1, 1980·Biomedical Mass Spectrometry·S J GaskellA W Pike
Jan 1, 1981·Annals of Clinical Biochemistry·B G JoyceD Riad-Fahmy
Mar 1, 1986·Annals of Clinical Biochemistry·A A IsmailM Wheeler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.