PMID: 6990558Apr 1, 1980Paper

A sensitive solid phase enzymeimmunoassay for norethisterone (norethindrone) in saliva and plasma

Steroids
A TurkesD Riad-Fahmy

Abstract

A sensitive, solid phase enzymeimmunoassay suitable for determining norethisterone in small aliquots of plasma (10 microliters) and saliva (100 microliters) has been developed. A solid phase antiserum raised against a norethisterone-11 alpha-hemisuccinyl/bovine serum albumin conjugate was prepared by coupling to cyanogen bromide activated cellulose. A norethisterone/horseradish peroxidase conjugate was used as enzyme label, o-phenylenediamine/hydrogen peroxide being the substrate for colour development. The assay had a lower limit of sensitivity of 3 pg/assay tube and satisfied accepted validation criteria. Norethisterone concentrations determined by enzymeimmunoassay and by a well established radioimmunoassay were in excellent agreement in both plasma (r = 0.993, n = 20) and saliva (r = 0.989, n = 15). Plasma and salivary norethisterone concentrations determined in healthy volunteers reached peak values at about 1 hour after administering a norethisterone-containing oral contraceptive preparation. The maximum values achieved in saliva (775--1430 pmol/l) were only approximately 3% of those observed in plama. Since salivary norethisterone concentrations reflected those in plasma, they may be useful in fertility control programme...Continue Reading

Citations

Oct 1, 1982·Journal of Steroid Biochemistry·G L HammondT Luukkainen
Jan 1, 1982·Contraception·I D NuttallD R Fahmy
Jan 1, 1986·Critical Reviews in Clinical Laboratory Sciences·R F Vining, R A McGinley
Oct 1, 1989·The Journal of Pharmacy and Pharmacology·G W Aherne, R Briggs
Dec 1, 1982·Clinical Endocrinology·J R PetersR Hall
Jan 1, 1990·Society for Applied Bacteriology Symposium Series·H S Tranter, R D Brehm

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