PMID: 6971103Mar 1, 1981Paper

A sensitive solid phase microradioimmunoassay for anti-double stranded DNA antibodies

Arthritis and Rheumatism
F Fish, M Ziff

Abstract

A sensitive solid phase microradioimmunoassay has been developed for measurement of antidouble stranded DNA (dsDNA) antibodies. In this procedure, advantage has been taken of the capacity of poly-L-lysine (PLL) to facilitate the binding of pure dsDNA to plastic surfaces. In the absence of PLL, binding did not occur. Diluted sera were incubated in PLL-treated dsDNA-coated microtitration trays and anti-dsDNA Ig measured using affinity purified 125I-anti-Ig of high specific activity. The synthetic DNA, poly dA-dT, was used as a model for dsDNA. In initial experiments, specific anti-DNA binding could not be demonstrated because of high background binding of patient Ig to PLL-treated surfaces. This was reduced by diluting test sera and anti-Ig in buffer containing 2% BGG and 1% BSA. Specificity of the assay for DNA was demonstrated by absorbing the anti-DNA activity on DNA-coated plastic. The binding of systemic lupus erythematosus (SLE) patient serum Ig to poly dA-dT coated trays did not diminish after digestion with nuclease S1, suggesting that the synthetic polymer is an appropriate model for dsDNA. Patient and normal sera were screened for anti-dsDNA activity using poly dA-dT as antigen. None of the 38 normal sera, 23 of 35 acti...Continue Reading

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Citations

Aug 28, 1990·Journal of Immunological Methods·W EmlenG Burdick
Apr 12, 1995·Journal of Immunological Methods·K GregoriusH I Elsner
Oct 1, 1984·Immunopharmacology·R H Weisbart, K Colburn
Oct 28, 1983·Journal of Immunological Methods·R L RubinE M Tan
Feb 1, 1985·The Journal of Experimental Medicine·D Wofsy, W E Seaman
Feb 28, 2016·Journal of Immunological Methods·Gabriella LakosThierry Dervieux
Aug 1, 1985·Clinical Immunology and Immunopathology·W S Yee, R H Weisbart
Mar 22, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zimu DengAnthony K Shum
Aug 1, 1989·European Journal of Immunology·H YakuraM Katagiri
Nov 28, 1985·Journal of Immunological Methods·K Yoshikawa, M Fukada

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