PMID: 6793074Aug 13, 1981

A sepharose derivative coupled with a leupeptin-like peptide aldehyde, glycylglycyl-L-argininal, and its use as an affinity adsorbent for trypsin

Biochimica Et Biophysica Acta
M NishikataS Ishii

Abstract

A Sepharose derivative containing a peptide aldehyde, glycylglycyl-L-argininal, the structure of which resembles that of leupeptin was prepared. It was a strong affinity adsorbent for trypsin (EC 3.4.21.4). Bovine trypsin showed higher affinity for this adsorbent at the optimum pH of catalysis (8.2) than at lower pH (5.0). This observation was in good agreement with the pH dependence of the interaction of leupeptin and trypsin (Kuramochi, H., Nakata, H. and Ishii, S. (1979) J. Biochem. 86, 1403-1410). Streptomyces griseus trypsin was also adsorbed while trypsinogen, alpha-chymotrypsin and TLCK-trypsin were not adsorbed. Though anhydrotrypsin, in which Ser-183 is converted to dehydroalanine, was not adsorbed, carbamoylmethylated (His-46) trypsin was adsorbed. Ser-183 proved to be essential for the binding. This adsorbent can also be used as a good tool to study the mechanism of action of leupeptin.

References

Apr 5, 1988·Journal of Molecular Biology·R J Read, M N James
Feb 5, 2000·Bioorganic & Medicinal Chemistry·T L VoyushinaV M Stepanov

Citations

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G D BrayerR C Thompson
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M WilchekY J Topper
Oct 17, 1972·Biochemical and Biophysical Research Communications·A ItoB Shimizu
Jun 19, 2009·Biology of Reproduction·Yashwanth RadhakrishnanFrank S French
Jan 3, 2013·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·David H SalzmanChad S Kessler
Jul 3, 2013·Cancer Immunology, Immunotherapy : CII·Bridget CharbonneauJames R Cerhan

Related Concepts

Histidine
Cathepsin G
Bos taurus
Alpha-chymotrypsin
Oligopeptides
Adsorption
Trypsin
Anhydrotrypsin
Aldehydes
Leupeptins

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