PMID: 6164994Apr 10, 1981Paper

A sequence from Drosophila melanogaster 18S rRNA bearing the conserved hypermodified nucleoside am psi: analysis by reverse transcription and high-performance liquid chromatography

Nucleic Acids Research
D C Youvan, J E Hearst

Abstract

The naturally occurring modified nucleoside 3-[3-amino-3-carboxypropyl]-1-methylpseudouridine (abbreviated am psi) is found in eukaryotic 18S rRNA. We localized am psi to sequence resolution in D. melanogaster 18S rRNA. This hypermodified base causes an absolute stop in cDNA elongation. The RNA sequence bearing am psi was determined by dideoxy-sequencing with reverse transcriptase. The rDNA coding for this part of the 18S rRNA was sequenced by the Maxam-Gilbert method. Together these two sequencing methods can be used to position the cDNA stop (am psi) in the rRNA sequence. Chemical evidence for the existence of am psi in this RNA sequence was obtained by high-performance liquid chromatography (HPLC) of 18S rRNA nucleosides from radioactive-labeled cells. L-[2-14C] methionine will selectively label am psi in eukaryotic 18S rRNA. Using HPLC, we found a single 14C-labeled nucleotide in digests of 18S rRNA. This nucleotide is in the RNA sequence bearing the cDNA stop since a restriction fragment which hybridizes to this sequence protects the modified base from RNase T1 digestion.

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D C Youvan, J E Hearst
Jul 1, 1979·European Journal of Biochemistry·J P Cecchini, R Miassod
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J BrosiusH F Noller
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·I B Dawid, P Botchan
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P L WollenzienJ E Hearst
Nov 1, 1970·In Vitro·G Echalier, A Ohanessian
Jan 25, 1980·Journal of Chromatography·C W GehrkeR W Zumwalt

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Citations

Sep 27, 2007·ACS Chemical Biology·Christine S ChowSantosh K Mahto
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A BartaE Kuechler
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J LaneN R Pace
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M UzanE Brody
Dec 26, 2006·The Journal of Biological Chemistry·Dmitry V LesnyakOlga A Dontsova
Aug 25, 1993·Nucleic Acids Research·R B Denman
Aug 25, 1995·Nucleic Acids Research·Y RheeJ Termini
Mar 29, 2007·Nucleic Acids Research·Petr V SergievOlga A Dontsova
Jun 1, 2012·Nucleic Acids Research·Dmitry E BurakovskyOlga A Dontsova
Jan 1, 1982·Analytical Biochemistry·D C Youvan, J E Hearst
May 1, 1983·Cell·J F Thompson, J E Hearst
Feb 20, 1987·Journal of Molecular Biology·A A Beauclerk, E Cundliffe
Feb 1, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tek N LamichhaneChristine S Chow
Feb 28, 2002·Methods : a Companion to Methods in Enzymology·B E Maden
Apr 5, 1984·Biochimica Et Biophysica Acta·J PietteA van de Vorst
Sep 14, 2018·Analytical Chemistry·Bei ChenYu-Qi Feng

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