A sequence motif conserved in diverse nuclear proteins identifies a protein interaction domain utilised for nuclear targeting by human TFIIS.

Nucleic Acids Research
Yan LingGarry T Morgan

Abstract

The three structural domains of transcription elongation factor TFIIS are conserved from yeast to human. Although the N-terminal domain is not needed for transcriptional activity, a similar sequence has been identified previously in other transcription factors. We found this conserved sequence, the LW motif, in another three human proteins that are predominantly nuclear localized. We investigated two examples to determine whether the LW motif is actually a dedicated nuclear targeting signal. However, in one of the newly identified proteins, hIWS1 (human Iws1), a region containing classic nuclear localization signals (NLS) rather than the LW motif was necessary and sufficient for nuclear targeting in HeLa cells. In contrast, human TFIIS does not possess an NLS and only constructs containing the LW motif were efficiently targeted to nuclei. Moreover, mutations in the motif could cause cytoplasmic accumulation of TFIIS and enabled a structure/function assay for the domain based on the efficiency of nuclear targeting. Finally, GST pull-down assays showed that the LW motif is part of a protein-binding domain. We suggest that the targeting role the LW motif plays in TFIIS arises from its more general function as a protein interaction...Continue Reading

References

May 1, 1995·The Journal of Cell Biology·H Siomi, G Dreyfuss
Aug 1, 1993·The Journal of Cell Biology·R S TumaM B Roth
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Mar 28, 1998·The Journal of Biological Chemistry·P B AllenP Greengard
Oct 6, 1998·Annual Review of Biochemistry·I W Mattaj, L Englmeier
Dec 16, 1998·The Journal of Cell Biology·M AlbertiniG Blobel
Jul 8, 1999·Current Opinion in Cell Biology·A M Krecic, M S Swanson
Oct 8, 1999·Molecular Biology of the Cell·M Bellini, J G Gall
Dec 10, 1999·Molecular Biology of the Cell·S G Pasion, S L Forsburg
Mar 21, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Wind, D Reines
May 16, 2000·The Journal of Biological Chemistry·V BoothC H Arrowsmith
May 29, 2000·Science·J D Lewis, D Tollervey
Jun 6, 2000·Trends in Biochemical Sciences·N Proudfoot
Oct 14, 2000·Annual Review of Cell and Developmental Biology·J G Gall
Mar 27, 2001·Nucleic Acids Research·F SantagatiA M Pedrini
Apr 5, 2002·Nature·Tom Maniatis, Robin Reed
Apr 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hugo CeulemansMathieu Bollen
May 8, 2002·The Journal of Biological Chemistry·Katsuhisa YamazakiTeijiro Aso
Sep 6, 2002·Biochimica Et Biophysica Acta·Rachel N Fish, Caroline M Kane
Sep 21, 2002·Molecular and Cellular Biology·Nevan J KroganJack F Greenblatt
Sep 24, 2002·Journal of Cell Science·Karla M Neugebauer
Oct 29, 2002·Nature Structural Biology·James L Manley
Mar 13, 2003·Molecular Biology of the Cell·Abigail J SmithGarry T Morgan
Apr 4, 2003·Current Opinion in Genetics & Development·Grant A Hartzog
Aug 19, 2003·Nature Reviews. Molecular Cell Biology·Angus I Lamond, David L Spector
Aug 29, 2003·Biochemical and Biophysical Research Communications·Kenji TamuraTeijiro Aso

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Citations

Dec 21, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Garry T Morgan
Feb 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Lei LiYanhai Yin
Feb 4, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Michael KochChristophe Romier
Jan 20, 2009·Journal of Molecular Biology·Marion GrasserKlaus D Grasser
Dec 23, 2006·Biochemical and Biophysical Research Communications·Zhangguo LiuShilai Bao
Oct 7, 2015·Frontiers in Plant Science·Irene MurgiaPiero Morandini
Sep 30, 2010·Journal of Molecular Biology·Venugopal PujariLaurie A Stargell
Aug 8, 2015·Nature Communications·Petr TesinaPavlína Řezáčová
Jan 5, 2018·Nucleic Acids Research·Sha LiLaurie A Stargell

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Methods Mentioned

BETA
interaction assay
PCR
transfection
pull-down
electrophoresis
NMR
X-ray
phage display
two-hybrid

Software Mentioned

PSORTII
Psi
BLAST

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