A sequence motif within chromatin entry sites directs MSL establishment on the Drosophila X chromosome.

Cell
Artyom A AlekseyenkoMitzi I Kuroda

Abstract

The Drosophila MSL complex associates with active genes specifically on the male X chromosome to acetylate histone H4 at lysine 16 and increase expression approximately 2-fold. To date, no DNA sequence has been discovered to explain the specificity of MSL binding. We hypothesized that sequence-specific targeting occurs at "chromatin entry sites," but the majority of sites are sequence independent. Here we characterize 150 potential entry sites by ChIP-chip and ChIP-seq and discover a GA-rich MSL recognition element (MRE). The motif is only slightly enriched on the X chromosome ( approximately 2-fold), but this is doubled when considering its preferential location within or 3' to active genes (>4-fold enrichment). When inserted on an autosome, a newly identified site can direct local MSL spreading to flanking active genes. These results provide strong evidence for both sequence-dependent and -independent steps in MSL targeting of dosage compensation to the male X chromosome.

References

Feb 21, 1997·Cell·H Amrein, R Axel
Mar 20, 1997·Nature·L B HerzingA Ashworth
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bas van SteenselHarmen J Bussemaker
Jul 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ling V SunKevin P White
Mar 16, 2004·Seminars in Cell & Developmental Biology·Mary F Lyon
Oct 27, 2004·PLoS Biology·Delphine Fagegaltier, Bruce S Baker
Jun 25, 2005·BMC Bioinformatics·Per StenbergJan Larsson
Nov 16, 2005·Annual Review of Genetics·John C LucchesiBarbara Panning
Feb 8, 2006·PLoS Genetics·Ina K DahlsveenPeter B Becker
Mar 21, 2006·Genes & Development·Gregor D GilfillanPeter B Becker
Mar 21, 2006·Genes & Development·Gaëlle LegubeAsifa Akhtar
Jul 18, 2006·Nucleic Acids Research·Timothy L BaileyWilfred W Li
Feb 27, 2007·Cell·Aaron D GoldbergEmily Bernstein
Feb 27, 2007·Cell·Bing LiJerry L Workman
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Johannes BischofKonrad Basler
Jul 3, 2007·Nature·Tarjei S MikkelsenBradley E Bernstein
Jul 10, 2007·PLoS Genetics·Eimear E HolohanRobert White
Nov 13, 2007·BMC Genomics·Miguel GallachIgnacio Marín
Dec 7, 2007·Genes & Development·Robert Schneider, Rudolf Grosschedl

❮ Previous
Next ❯

Citations

Apr 21, 2011·Chromosoma·Per Stenberg, Jan Larsson
Mar 25, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Sevinc Ercan, Jason D Lieb
Jul 25, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Beatriz Vicoso, Doris Bachtrog
Oct 6, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Erinc Hallacli, Asifa Akhtar
Oct 6, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Györgyi CsankovszkiKarishma S Collette
Oct 24, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jamila I Horabin
Nov 20, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Claudia Keller, Marc Bühler
Jun 2, 2010·International Journal of Obesity : Journal of the International Association for the Study of Obesity·M ZeydaT M Stulnig
Sep 9, 2009·Nature Reviews. Genetics·Peter J Park
May 15, 2010·Nature Reviews. Immunology·Frédéric GeissmannGwendalyn J Randolph
Oct 11, 2011·Nature Reviews. Immunology·Ajay ChawlaY P Sharon Goh
Jan 24, 2012·Nature Reviews. Molecular Cell Biology·Eric J Wagner, Phillip B Carpenter
Aug 4, 2009·Nature Structural & Molecular Biology·Marnie E GelbartMitzi I Kuroda
Jul 27, 2010·Nature Structural & Molecular Biology·Daesung KimSepideh Khorasanizadeh
Jan 11, 2011·Nature Structural & Molecular Biology·Jan KadlecAsifa Akhtar
Dec 6, 2012·Nature Structural & Molecular Biology·Catherine A MusselmanTatiana G Kutateladze
Jan 9, 2013·Nature Structural & Molecular Biology·Charlotte I WangMitzi I Kuroda
Jan 7, 2009·Nature Structural & Molecular Biology·Alison M Hosey, Marjorie Brand
Dec 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Matthew D SimonRobert E Kingston
Jul 29, 2009·The Journal of Heredity·Danielle VermaakHarmit S Malik
May 10, 2011·Nucleic Acids Research·Anna-Mia JohanssonJan Larsson
Oct 15, 2010·Cold Spring Harbor Perspectives in Biology·Jennifer C Chow, Edith Heard
Jul 23, 2013·Genes & Development·Marcela M L SorucoErica Larschan
Nov 4, 2009·Genes & Development·Charlotte Grimaud, Peter B Becker
Nov 16, 2013·Science·Christopher E Ellison, Doris Bachtrog
Nov 16, 2013·Science·Edward B Chuong, Cédric Feschotte
Feb 4, 2009·Molecular and Cellular Biology·Timothy A Blauwkamp, Gyorgyi Csankovszki
Jul 29, 2010·BMC Bioinformatics·Shouyong PengPeter J Park
Jan 23, 2009·BMC Genomics·Philippe LefrançoisMichael Snyder
Aug 4, 2012·BMC Genomics·Zhong Wang, Huntington F Willard
Dec 18, 2009·BMC Research Notes·Nicolas Buisine, Laurent Sachs

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.