Abstract
In this paper, a new chemiluminescent plant tissue-based biosensor for diamine detection was presented by employing sequential injection analysis (SIA), which facilitates precise fluidic handling and lower consumption of sample and reagents. Pea-seedling tissue acted as the molecular recognition element and was packed in a mini-PTFE column and further incorporated in the SIA system. The analysis of diamines, such as putrescine and cadaverine, is based on an enzymatic conversion which takes place in the plant tissue column to produce hydrogen peroxide. The formed hydrogen peroxide was detected by a chemiluminescence reaction involving luminol and Co(2+). Under the optimal conditions, the linear calibration graphs were obtained within 0.2-80 microM (putrescine) and 0.5-100 microM (cadaverine). The detection limits of 0.03 and 0.06 microM were achieved for putrescine and cadaverine, respectively, along with the relative standard deviations of 2.14% and 3.08% (n=11) and a sampling frequency of 40 h(-1). The present biosensor has been used for the analysis of diamine in fish samples with an acceptable accuracy.
References
Jan 15, 1979·Analytical Biochemistry·T A Smith
Mar 2, 1999·Protein Expression and Purification·F VianelloA Rigo
Mar 18, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·M Y KhuhawarM I Bhanger
Jan 21, 2000·Biosensors & Bioelectronics·M Wimmerová, L Macholán
Aug 19, 2000·Journal of Chromatography. a·J O Fernandes, M A Ferreira
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·M VenzaD Teti
Apr 9, 2002·Analytical and Bioanalytical Chemistry·Jana Lange, Christine Wittmann
Apr 18, 2002·Biosensors & Bioelectronics·Baoxin LiYan Jin
Jul 12, 2002·Journal of Pharmaceutical and Biomedical Analysis·N A SantagatiG Ronsisvalle
Apr 7, 2004·Journal of Chromatography. a·A L CinquinaF Abballe
May 1, 2004·Journal of Chromatography. a·S Meseguer LloretP Campíns Falcó
Aug 4, 2005·Biosensors & Bioelectronics·Fangqiong WuChengzhi Huang
Oct 31, 2008·Talanta·Seung-Hyun LeeJong Il Rhee
Citations
Feb 14, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yufei HuChunyan Yang
Sep 10, 2008·Luminescence : the Journal of Biological and Chemical Luminescence·Fakhr Eldin O SulimanSalama B Salama
Mar 15, 2011·Analytica Chimica Acta·Cristina I C SilvestreJosé L F C Lima
May 23, 2008·Biotechnology Advances·M CampàsR Rouillon
Dec 20, 2011·Journal of Separation Science·Juanjuan LuJinghua Yu